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. 2023 Jul 13;14(7):418. doi: 10.1038/s41419-023-05954-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Flow cytometry of GFP(IL-10)⁺ B cells in the lungs. B Proportion and number of GFP(IL-10)⁺ B cells as a proportion of live events. The lung total number of cells was then multiplied by the ratio to give the total GFP(IL-10)⁺ B cells count. (n = 8). C The number of different GFP (IL-10)-secreting cell subsets in the lungs (n = 9–10); DC dendritic cells, MF macrophages, MO monocytes, EC epithelial cells. D The proportion of IL-10-secreting subpopulations in the lungs of IL-10-GFP mice during LPS exposure (n = 9–10). E Immunofluorescence in the lungs of mice after four days of LPS exposure and the proportion of IL-10-secreting B cells (n = 3). Scale bars, 20 μm. Representative one of two independent experiments. Student’s t-test was used for the statistical analyses.