Fig. 3. Electrostatic, hydrophobic, and domain swapping maintains interactions between lattice sheets.

a Polyhedrin molecules which form major interfaces between the sheets of the polyhedra. The reference polyhedrin dimer (blue/red), with polyhedrin from the sheets in front (yellow/purple) and behind (green) are shown. A simplified scheme (right side) showing the relative arrangement of polyhedrin around the three-fold crystallographic axis (pink triangle), two-fold axis (pink arrow), and the change in direction of each sequential sheet in the lattice. b Detailed view of the domain swapping interactions annotated with secondary structure names. The crystallographic 2-fold axis is shown (pink ellipse). c SDS-PAGE of recombinant crystals treated with NaH₂CO₃ or water under reducing (R) or non-reducing (NR) conditions. Samples were prepared twice and a representative image shown.