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Stability of recombinant ToNV crystals
| Stress type | Condition | Duration (h) | Diffraction |
|---|---|---|---|
| Chaotropic agent | 8 M Urea | 24 | Yes |
| Detergent | 10% w/v SDS | 24 | Dissolved |
| Acid | 1 M HCl | 24 | No |
| Base | 20 mM NaH2CO3 pH 10.5 | 3 or 24 | Dissolved |
| Solvent | 100% Ethanol | 3 or 24 | Yes |
| High molarity | 4 M NaCl | 24 | Yes |
| Oxidising agent | 1% v/v H2O2 | 24 | Yes |
| Reducing agent | 10 mM Dithiothreitol | 24 | Yes |
| Physical stress | 90 °C temperature | 3 | Yes |
| Freeze-thaw | – | Yes |
