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The mixture solution with different compositions for lipase inhibitory activity determination.
| Treatments | Fractionated compounds | pNPP solution | Lipase | A405nm |
|---|---|---|---|---|
| Samples | √ | √ | √ | As |
| Enzyme blank | √ | √ | X | Ab |
| Sample blank | B | √ | √ | At |
| Blank | B | √ | X | Ac |
Note: √ Indicates that included in the reaction mixture; X indicates that not included in the reaction mixture; and B indicates only buffer.
