Figure 8.
Renal fibrosis analysis in all groups. (A) Coloration in Picro Sirius Red (PSR), (B) immunostaining of anti-TGF-β, (C) immunostaining of anti-α-SMA, (D) quantification of immunostaining (pixel/µm2) for anti-TGF-β and (E) quantification of immunostaining (pixel/µm2) for anti-α-SMA. Arrows indicate positive immunostaining mainly in tubules. An increase in collagen deposition was observed in the HFD group compared to the other groups. In an immunohistopathological study, a significant increase in TGF-β and anti-α-SMA levels was observed in the HFD group when compared to the CD and CD + CO groups. In the HFD + CO group, a significant decrease in this microfilament was observed when compared to the HFD group. (a–c) represent p < 0.05 in relation to the CD, CD + CO and HFD + CO groups, respectively. n = 6 for all experimental groups. Calibration bar: 100 µm (anti-TGF-β) and 50 µm (anti-α-SMA and PSR).