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. 2023 May 1;24(8):914–931. doi: 10.1111/mpp.13338

FIGURE 3.

FIGURE 3

AvrLm10A_like1, AvrLm10B_like1, AvrLm10A_like2, and AvrLm10B_like2 co‐localize in Nicotiana benthamiana cells, but only AvrLm10A_like2 and AvrLm10B_like2 physically interact. (a) Single‐plane confocal images of N. benthamiana epidermal leaf cells expressing AvrLm10A_like1‐GFP, AvrLm10B_like1‐RFP, AvrLm10A_like2‐GFP, and AvrLm10B_like2‐RFP at 48 h postinfiltration of Agrobacterium tumefaciens. (b) Proteins were extracted 48 h after infiltration and analysed by immunoblotting with anti‐RFP (α‐RFP) antibodies (Input). Immunoprecipitation was performed with anti‐RFP beads (IP RFP) and analysed by immunoblotting with anti‐RFP antibodies to detect AvrLm10B_like1‐RFP, AvrLm10B_like2‐RFP, and RFP, and with anti‐GFP (α‐GFP) antibodies for the detection of co‐immunoprecipitated proteins.