Figure 2.

Piceatannol attenuated PC-12 cell death and autophagy induced by H₂O₂. (A) PC-12 cells were pretreated with piceatannol for 24 h, exposed to 250 μM H₂O₂ for another 24 h. Then cells were stained with MDC and PI fluorescence probe and detected by flow cytometer. (B) The protein expression level of LC3B II/I and ATG5 measured by Western blotting. PC-12 cells were incubated with piceatannol for 24 h, then treated with rapamycin (Rapa) for 4 h and H₂O₂ for another 24 h. (C) The MDC/PI probe staining observed by fluorescence microscope (Magnification, ×100; Scale bar = 100 μm). (D) The protein expression of LC3B II/I and ATG5 measured by Western blotting assay. (E) The cell viability detected by CCK8 assay. The data for the experiments were represented as the means ± standard deviation (SD). Results marked with the same letters were not significantly different (p < 0.05).