Figure 3.

Piceatannol improved mitochondrial function of PC-12 cells by inhibiting autophagy. PC-12 cells were pretreated with piceatannol for 24 h, then treated with rapamycin for 4 h and H₂O₂ for another 24 h. (A) Mitochondrial content observed by fluorescence microscope (Magnification, ×100; Scale bar = 100 μm). (B) Mitochondrial membrane potential observed by flow cytometer. (C) The protein expression level of PGC-1α, Complex IV and TFAM measured by Western blotting assay. The data for the experiments were represented as the means ± standard deviation (SD). Results marked with the same letters were not significantly different (p < 0.05).