Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 1;10(8):nwad165. doi: 10.1093/nsr/nwad165

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Spatial reorganization of focal adhesions and cytoskeletal in response to cell shape changes in 3D micropatterns. (a) Representative confocal images of vinculin (red) identifying focal adhesions, F-actin (green) and nucleus (blue) for the single cell in 3D micropatterns. Scale bar, 50 μm. (b) Quantification of the total focal adhesions area of cells confined in 3D micropatterns. (c) Fluorescence images of F-actin filament staining for MSCs cultured in 3D micropatterns. Scale bar, 50 μm. (d) Quantification of fluorescence intensity of F-actin in different 3D micropatterns. (e and f) F-actin fibers thickness in 3D micropatterns evaluated by fluorescence intensity. (g) Representative fluorescence images of myosin staining of MSCs cultured in 3D micropatterns. Scale bar, 50 μm. (h) Quantification of myosin intensity of MSCs cultured in 3D micropatterns. The data are represented as the mean ± SD, n = 30–40 cells per condition, ** p < 0.01, *** p < 0.001.