(A) UMAP plot of snRNAseq profiles from TI of selected NEPTUNE participants found to have elevated TNF activation scores (TNF high) and low to moderate TNF activation scores (TNF low) and (B) Single nuclear cluster expression of CCL2 and TIMP1 by TNF activation status. Cell type specific markers were based on Lake et al, 202128.
Abbreviations: ATL = ascending thin limb cells; DCT = distal convoluted tubule cells; DTL = descending thin limb cells; EC-GC = glomerular endothelial cells; FIB = fibroblast cells; Immune = several types of immune cells; PC/IC = principal cells / intercalated cells; POD = podocytes; PT = proximal tubule cells; TAL = thick ascending limb cells.