FIGURE 10.
Methanol fed-batch with ALE_Lh_LDH strain. Cultivation of an evolved O. polymorpha strain overproducing an LDH of Lactobacillus helveticus using urea (A,B,C) or ammonium sulfate (D,E,F) as N source in the cultivation medium. Development of lactate titer (A, D; blue squares), optical density (A, D, OD600 nm, green dots), methanol concentration (B, E, red dots) and pH (C, F, black dots). Cultivations were done in Verduyn medium with 0.5% MeOH as initial carbon source. Additional pulses of 0.5% or 2% methanol were supplied to the culture. Experiments were performed in 500 mL shake flasks. Error bars represent the standard deviation of three biological replicates.