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Growth and lactate production of single colonies of evolved O. polymorpha Lh_LDH population with methanol as carbon source. Development of biomass formation (A) and lactate titers at the end of the cultivation (B). The unevolved reference strain (Lh_LDH) was cultivated in biological triplicates, while the evolved colonies (ALE 1–12) were cultivated in single replicates. Cultivation in BioLector microtiter plates in Verduyn medium with 0.5% methanol as carbon source.
