TABLE 2.
Single-nucleotide polymorphisms in the evolved O. polymorpha ALE_Lh_LDH strain compared to the unevolved Lh_LDH strain.
| SNP No. | Nt position | Gene | Sequence change | Protein change |
|---|---|---|---|---|
| 1 | Contig 3, 213215 | Methanol oxidase (MOX) | TAC → CAC | Tyr → His |
| 2 | Contig 4, 468138 | Intergenic, Located between ORF for putative kynureninase & putative sulfate permease | G → A | |
| 3 | Contig 1, 575572 | Downstream of LDH | G → A | |
| 4 | Contig 1, 575780 | Downstream of LDH | AT → GC | |
| 5 | Contig 2, 1240617 | Putative ORF | TGT → TCT | Cys → Ser |
| 6 | Contig 2, 1240654 | Putative ORF | ATG → TTG | Met → Leu |