Table 2.

Quadruple quantitative polymerase chain reaction mixture components

Reagents	Volume (µL)
2× HyperProbe Mixture	12.5
B646L-F (20 µM)	0.3
B646L-R (20 µM)	0.3
B646L-P (10 µM)	0.2
EP402R-F (20 µM)	0.2
EP402R-R (20 µM)	0.2
EP402R-P (10 µM)	0.4
A137R-F (10 µM)	0.4
A137R-R (10 µM)	0.4
A137R-P (10 µM)	0.6
MGF505-3R-F (10 µM)	0.2
MGF505-3R-R (10 µM)	0.2
MGF505-3R-P (10 µM)	0.1
DNA	3
ddH2O	6
Total	25

ddH₂O, double-distilled water