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. 2023 Jul 13;21:224. doi: 10.1186/s12951-023-01961-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — In vitro anti-tumor effect of anti-miRNA/FePS@PPF. a Images of HOS cells treated with FITC-labeled FePS@PPF and b Cy5.5-anti-miR-19a/FePS@PPF for 6 h (scale bar is 50 μm) obtained from confocal microscope. c Relative cell viabilities after incubation with FePS@PPF with various concentrations at 48 h. d Relative cell viability in the group of PBS (Control), anti-miR-NC/FePS@PPF and anti-miR-19a/FePS@PPF with or without NIR (FePS@PPF concentration of 25 µg/mL). e Fluorescence live (green)/dead (red) images (scale bar is 200 μm). f Flow cytometry analysis of apoptotic/necrotic cells and g the statistics of relative percentage of tumor cell apoptosis. h, i Western blot analysis of PTEN, AKT and p-AKT expression after different treatments for 48 h. **p < 0.01, G3, G5 and G6 compared to Control (G1)