Figure 4.

The immunogenicity of Ad2-ASFV after prime-boost vaccination via IM + IN administration in mice. (A) Schematic of the prime-boost immunization scheme in mice. Mice received a second dose of the same vaccine 56 days later. The red blood drop symbols represent the time points when serum samples were collected. The mice were sacrificed 84 days following the prime vaccination. (B–E) The IgG antibodies against p30 (B), p54 (C), CD2v (D), and p72 (E) at the indicated time points postvaccination were assessed by ELISA. (F) ELISA was used to assess IgA antibodies against the p30, p54, CD2v, and p72 proteins in BALFs. (G) IFN-γ ELISpot was used to detect the number of antigen-specific IFN-γ-secreting cells per million splenic lymphocytes in mice. The data represent the results of three independent experiments. Comparisons using the student’s t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.