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. Author manuscript; available in PMC: 2024 May 17.
Published in final edited form as: Cell Syst. 2023 May 9;14(5):363–381.e8. doi: 10.1016/j.cels.2023.04.002

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
Yeast Extract BD Difco DF0127179
Peptone BD Difco DF0118170
G-418 RPI G64000-1.0
D(+) Glucose Thermo Fisher AAA168280E
Hygromycin B RPI H75020-1.0
CellTracker Blue CMAC Dye Invitrogen C2110
MitoTracker Red CMXRos Invitrogen M7512
Tunicamycin Sigma SML1287-1ML
Fluconazole Sigma PHR1160-1G
Sodium Chloride Spectrum S1240-1KG
Hydroxyurea Thermo Scientific A10831.14
Hydrogen Peroxide Fisher Scientific H323-500
DMSO Amresco 0231-500ML
Poly(ethylene-glycol) 3350 Sigma P4338-500G
ssDNA Life Technologies 15632011
Lithium Acetate dihydrate Sigma L4158-100G
Deposited data
Deletion screen colony growth images This paper https://doi.org/10.6084/m9.figshare.21741434.v1
Ribosome profiling analysis results This paper https://doi.org/10.6084/m9.figshare.22312729.v1
C-SWAT collection Meurer et al.60 Supplementary Table 2
YeastRGB collection Dubreuil et al.61 Yeastrgb.org
CYCLoPs collection Ko et al.59 https://thecellvision.org/cyclops/
Saccharomyces cerevisiae S288C reference genome sequence R64.2.1 Saccharomyces genome database http://sgd-archive.yeastgenome.org/sequence/
S. paradoxus genome Liti et al. 200980 http://www.saccharomycessensustricto.org/
S. arboricolus genome Liti et al. 201381 GCF_000292725.1
S. jurei genome Naseeb et al. 201882 GCA_900290405.1
S. mikatae, S. bayanus var. uvarum, S. bayanus var. bayanus, and S. kudriavzevii genome Scannell et al. 201151 http://www.saccharomycessensustricto.org/
TIF-seq data Pelechano et al. 201447 GSE39128
S. cerevisiae strain genomes Peter et al. 201840 http://1002genomes.u-strasbg.fr/files/
Budding yeast genomes Shen et al. 201841 https://y1000plus.wei.wisc.edu/data
Experimental models: Organisms/strains
Saccharomyces cerevisiae: BY4741 Dharmacon YSC1048
Saccharomyces cerevisiae: BY4741, deletion collection Dharmacon YSC1053
Saccharomyces cerevisiae: BY4741, ORF::KanMx (mini collection with the 49 nORFs and 3 cORFs deleted) This paper Wacholder 2023, deletion collection, see Table S10
Saccharomyces cerevisiae: BY4741, ORF-mNG:HYG (mini collection with the selected ORFs tagged with mNeonGreen) This paper Wacholder 2023, mNG collection, see Table S10
Saccharomyces cerevisiae: BY4741, YDL204W-A(wt):HYG This paper yARC0602
Saccharomyces cerevisiae: BY4741, YDL204W-A(ATG→AAG):HYG This paper yARC0601
Saccharomyces cerevisiae: BY4741, YBR196C-A(wt):HYG This paper yARC0604
Saccharomyces cerevisiae: BY4741, YBR196C-A(ATG→AAG):HYG This paper yARC0603
Saccharomyces cerevisiae: BY4741, YDR073C-A(wt):HYG This paper yARC0606
Saccharomyces cerevisiae: BY4741, YDR073C-A(ATG→AAG):HYG This paper yARC0605
Saccharomyces cerevisiae: BY4741, YGR016C-A(wt):HYG This paper yARC0608
Saccharomyces cerevisiae: BY4741, YGR016C-A(ATG→AAG):HYG This paper yARC0607
Saccharomyces cerevisiae: BY4741, YJL077C(wt):HYG This paper yARC0610
Saccharomyces cerevisiae: BY4741, YJL077C(ATG→AAG):HYG This paper yARC0609
Saccharomyces cerevisiae: BY4741, YNL040C-A(wt):HYG This paper yARC0612
Saccharomyces cerevisiae: BY4741, YNL040C-A(ATG→AAG):HYG This paper yARC0611
Saccharomyces cerevisiae: BY4741, YPR096C(wt):HYG This paper yARC0614
Saccharomyces cerevisiae: BY4741, YPR096C(ATG→AAG):HYG This paper yARC0613
Saccharomyces cerevisiae: BY4741, YDL204W-A(wt):HYG, pAG-GPD-ccdB1-KanMx This paper yARC0831
Saccharomyces cerevisiae: BY4741, YDL204W-A(ATG→AAG):HYG, pAG-GPD-ccdB1-KanMx This paper yARC0842
Saccharomyces cerevisiae: BY4741, YDL204W-A(ATG→AAG):HYG, pAG-GPD-YDL204W-A-KanMx This paper yARC0848
Recombinant DNA
Plasmid: pAG-GPD-ccdB1-KanMx This paper pARC0112
Plasmid: pAG-GPD-YDL204W-A-KanMx This paper pARC0300
Software and algorithms
Code for analyses conducted This paper https://doi.org/10.5281/zenodo.7474228
Code for analyzing images of colonies on plates This paper https://doi.org/10.5281/zenodo.7760846
R version 4.12 R https://www.r-project.org/
BLAST 2.9.0+ National Library of Medicine https://blast.ncbi.nlm.nih.gov/blast/Blast.cgi
Ontologizer 2.0 Bauer et al. 200883 http://ontologizer.de/
water EMBOSS https://www.ebi.ac.uk/Tools/psa/emboss_water/
MUSCLE 3.8.31 Edgar 200484 https://www.drive5.com/muscle/