Table 2.

Microvessel density assessment

Ref.	MVD assessment method	Antibody	Magnification used	Spots examined	Blinded reading	Observers	Separate count for intra/peritumoral vessels	MVD cut off
Chen et al[22]	Light microscopy, immunohistochemistry	CD31	10X; 20X; 100X	3	N/A	N/A	N/A	15/HPF
Imamura et al[8]	Light microscopy, immunohistochemistry, slight modification of Horak et al technique	CD31	40X; 200X	10	Yes	2	N/A	7/0.95 mm²
Takahashi et al[23]	Light microscopy, immunohistochemistry	CD31	40X; 100X; 400X	3	N/A	N/A	N/A	19/HPF
Waengertner et al[24]	Light microscopy, immunohistochemistry, modified Chalkley method	CD31	200X	3 to 5	N/A	N/A	N/A	6 vessels
Wang et al[9]	Light microscopy, immunohistochemistry	CD31	200X	4	Yes	2	N/A	10.54/200HPF
Zhao et al[25]	Light microscopy, immunohistochemistry, Weidner technique	CD34	100X; 200X	5	N/A	N/A	N/A	N/A

MVD: Microvessel density; N/A: Not applicable.