Figure 3.

Dwell time measurements of the R24 dimer at different forces. (a) The relationship between bead height and time for the FLNC R24 dimer is shown, with each force–jump cycle depicted in a different color for forces of 8 and 14 pN. The zero time point marks the moment when the applied force transitions from 1 pN to the focused forces of 3, 8, and 14 pN. The right insets in each panel display the red and blue blocks representing the dissociated, associated, and combined dissociated and one unfolded R24 domain states of the FLNC R24 dimer. The double arrows indicate the recorded dwell time in each cycle. (b) Histogram of dwell times is shown, along with the fitted curve that was used to calculate the lifetime of the FLNC R24 dimer at the focused forces (represented as τ) through a single-exponential decay.