Table 4.
The Levels of Target mRNA Detected Using PCR After Drug Intervention
| Group | IL-6 | IL-23A | K17 |
|---|---|---|---|
| Model | 2.69 ± 0.96 | 2.56 ± 0.53 | 2.73 ± 0.62 |
| CHI | 2.90 ± 0.58 | 1.98 ± 0.17 | 3.40 ± 0.40 |
| GEN | 0.96 ± 0.22 | 1.30 ± 0.18 | 1.82 ± 0.26 |
| CHI-GEN | 0.60 ±0.05 | 0.75 ± 0.06 | 1.58 ± 0.24 |
| MTX | 1.03 ± 0.11 | 0.70 ± 0.15 | 1.19 ± 0.09 |
Abbreviations: GEN, Gentiopicrin; CHI, Chitosan; CHI-GEN, Gentiopicroside-loaded chitosan nanoparticles; FITC-CHI, FITC-loaded chitosan nanoparticles; HaCaT, human-immortalized keratinocytes; TNF-α, tumor necrosis factor-α; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide;K17, keratin 17; VEGF, vascular endothelial growth factor; Interleukin 6, IL-6; Interleukin 23A, IL-23A; Interleukin 17A, IL-17A; qRT-PCR, quantitative Real-time Polymerase Chain Reaction; IMQ, Imiquimod; PASI, psoriasis area severity index; H&E, hematoxylin-eosin; MEM, Minimum essential medium; FBS, fetal bovine serum; TRY, trypsin-EDTA solution; MTX, methotrexate; TAC, Tacrolimus; PBS, Phosphate-buffered saline; DMSO, Dimethyl sulfoxide; PVDF, Polyvinyl difluoride; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; TEM, transmission electron microscopy; DLS, dynamic light scattering.