Extended Data Table. 1.
Plasmids used in this study.
| plasmids | Figure index | Constructs (backbone) | Note on construct |
|---|---|---|---|
| 1 | Fig 1b–d | pCAG-tdTom | Addgene# 83029 |
| 2 | pCAG-BFP-sesRNA-2a-eGFP | Backbone from Addgene# 100844, Gcamp6s was replaced with BFP-sesRNA-2a-eGFP | |
| 3 | Fig 1e–g | pTRE-BFP-2a-Cheta-pPGK-puro-2a-rtTA | Backbone from Addgene# 71782, BFP-2a-Cheta was inserted after TRE elements |
| 4 | pCAG-BFP-2a-Cheta | Backbone from Addgene# 100844, Gcamp6s was replaced with BFP-2a Cheta | |
| 5 | pCAG-mCherry-sesRNA-2a-eGFP | Backbone from Addgene# 100844, Gcamp6s was replaced with mCherry-sesRNA-2a-eGFP | |
| 6 | Fig 1h–i | pCAG-tdTom | |
| 7 | pCAG-spacer(2.1kb)-2a-sesRNA-2a-eGFP | from plasmids#5 spacer sequence was from CasRx coding regions of Addgene# 109049, 2.1kb from ATG start | |
| 8 | pCAG-ADAR2-sesRNA-2a-eGFP | from plasmids#5, ADAR2 sequence was from Addgene# 117929 | |
| 9 | Fig 1j–k | pCAG-tdTom | |
| 10 | pCAG-ClipF-sesRNA-2a-eGFP | from plasmids#5, ClipF sequence was from Addgene# 174554 | |
| 11 | pCAG-ADAR2-sesRNA-2a-eGFP | ||
| 12 | Fig 2a–d | pCAG-BFP-sesRNA-2a-eGFP | |
| 13 | pEF1a-Cheta-tdTom | Addgene# 37756 | |
| 14 | Fig 2e | pCAG-BFP-sesRNA-2a-ffLuc | from plasmids#8, ffLuc sequence was from Addgene# 71394 |
| 15 | Fig 2f | pEF1a-Cheta-tdTom | |
| 16 | pCAG-BFP-sesRNA-2a-ffLuc | ||
| 17 | Fig 2g–i | pEF1a-Cheta-tdTom | |
| 18 | pCAG-BFP-sesRNA-2a-eGFP | ||
| 19 | Fig 2j–m | pEF1a-Cheta-tdTom | |
| 20 | pEF1a-Cheta | From Addgene# 37756, Cheta-tdTom was replaced with Cheta | |
| 21 | pCAG-tdTom | ||
| 22 | pCAG-BFP-sesRNA-2a-eGFP | ||
| 23 | pCAG-BFP-sesRNA-2a-ffLuc | ||
| 24 | Fig 3b–e | pCAG-BFP-sesRNA-2a-eGFP | |
| 25 | Fig 3f–h | pCAG-ClipF-sesRNA-tTA | Backbone from Addgene# 112668,rtTA was replaced with ClipF-sesRNA-tTA |
| 26 | Fig 4a–c | pAAV-hSyn-mCherry-sesRNA-2a-smFlag-2a-tTA2 | smFlag from Addgene #59756 |
| 27 | pAAV-TRE3g-mNeonGreen | ||
| 28 | pAAV-TRE3g-GCaMP6s | GCaMP6s from Addgene #100844 | |
| 29 | pAAV-TRE3g-ChRger2-eYFP | ChRger2-eYFP from Addgene #127239 | |
| 30 | pAAV-TRE3g-eGFP | TRE3g backbone was kindly gifted from Nelson lab at Brandeis University | |
| 31 | Fig 5 | pAAV-hSyn-ClipF-sesRNA-2a-smFlag-tTA2 | ClipF sequence was from Addgene# 174554 |
| 32 | pAAV-TRE3g-mNeonGreen | TRE3g backbone was kindly gifted from Nelson lab at Brandeis University | |
| 33 | Extended Data Fig 1b, c | PKG-tdTom | |
| 34 | CAG-sesRNA-2a-eGFP | ||
| 35 | PKG-Empty | ||
| 36 | Extended Data Fig 1e, f | PKG-tdTom | |
| 37 | CAG-sesRNA-2a-tTA2 | ||
| 38 | TRE3g-ffLuc | TRE3g backbone was kindly gifted from Nelson lab at Brandeis University | |
| 39 | Extended Data Fig 1 g, h | pCAG-spacer-sesRNA-2a-eGFP | Spacer sequence was from CasRx coding regions of Addgene# 109049, different length was used from ATG start as indicated |
| 40 | pCAG-tdTom | ||
| Extended Data Fig 1 i, j | pCAG-BFP-sesRNA-2a-tTA2 | ||
| TRE3g-eGFP | |||
| 41 | Extended Data Fig 2 | pCAG-BFP-sesRNA-2a-Cas9–2a-eGFP | Cas9 from addgene# 62988 |
| 42 | pCAG-BFP-sesRNA-2a-tcaCasp3-TEVp | tcaCasp3-TEVp from addgene# 45580 | |
| 43 | Extended Data Fig 3c–f | pTRE-BFP-2a-Cheta-pPGK-puro-2a-rtTA | |
| 44 | pCAG-BFP-2a-Cheta | ||
| 45 | pCAG-mCherry-sesRNA-2a-eGFP | ||
| 46 | Extended Data Fig 4 | CMV-ADAR1-p110 | From addgene# 117928, GFP coding region was deleted |
| 47 | CMV-ADAR1-p150 | From addgene# 117927, GFP coding region was deleted | |
| 48 | Extended Data Fig 9 e, f | pCAG-BFP-2a-TargetGene | Target Gene was amplified from mice genomic DNA, Cheta was used as a control. Details in Extended Data Fig.10 |
| 49 | pCAG-mCherry-sesRNA-2a-eGFP | ||
| 50 | Extended Data Fig 9 h–k | pAAV-hSyn-mCherry-sesRNA-2a-smFlag-2a-tTA2 | |
| 51 | pAAV-TRE3g-mNeonGreen | ||
| 52 | Extended Data Fig 10 | pAAV-hSyn-ADAR2–2a-sesRNA-tTA2 | |
| 53 | pAAV-TRE3g-mRuby3 | ||
| 54 | pAAV-TRE3g-eGFP | ||
| 55 | Extended Data Fig 12, 15 | pAAV-hSyn-mCherry-sesRNA-2a-smFlag-2a-tTA2 | |
| 56 | Extended Data Fig 16 | pAAV-hSyn-ClipF-sesRNA-2a-smFlag-FlpO | FlpO from previous study (Matho et al Nature 2011) |
| 57 | Extended Data Fig 16 | pAAV-hSyn-ClipF-sesRNA-2a-smV5-tTA2 | smV5 from addgene # 71817 |