Extended Data Table. 2.
SesRNA used in this study.
| Figure Index | sesRNA name | Target gene | sesRNA position | Leng th | Stop Codon No. | sesRNA sequence |
|---|---|---|---|---|---|---|
| Fig. 1–2, Extended Data Fig. 1–6 | tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 1, Extended Data Fig. 1–6 | Ctrl | Not Target | N/ | 195 | 1 | aggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctactagcttcctctacggcagcaagaccttca tcaaccacacccagggcatccccgacttcttcaagcagtccttccctgagggcttcacatgggagagagtcaccacata |
| Fig. 1, Extended Data Fig. 1–6 | Cheta | Cheta | CDS | 228 | 1 | agccctcggggaaggacagcttcttgtaatcggggatgtcggcggggtgcttcacgtacgccttggagccgtacaggaactggggggacaggatgtcccaggcgaagggcagg gggccgcccttggtcaccttcagcttagcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcatggagccctccatgcgcacct |
| Fig. 2a | 25nt-tdT | tdTomato | CDS | 25 | 1 | cctcccagcccatagtcttcttcta |
| Fig. 2a | 50nt-tdT | tdTomato | CDS | 50 | 1 | gctcggtggaggcctcccagcccatagtcttcttctgcattacggggccg |
| Fig. 2a | 100nt-tdT | tdTomato | CDS | 100 | 1 | tcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttcttctgcattacggggccgtcgggggggaagttggtgccgca |
| Fig. 2a | 150nt-tdT | tdTomato | CDS | 150 | 1 | gggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttcttctgcattacggggccgtcgggggggaagttggtg ccgcgcatcttcaccttgtagatcagcgtgccgt |
| Fig. 2a | 300nt-tdT | tdTomato | CDS | 300 | 1 | Gcagttgcacgggcttcttggccatgtagatggtctggaactccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggt acaggcgctcggtggaggcctcccagcccatagtcttcttctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg aggagtcctgggtcacggtcaccagaccgccgtcctcgaagttcatcacgcgctcccactggaagc |
| Fig. 2a | 400nt-tdT | tdTomato | CDS | 400 | 1 | tgtccagcttggtgtccacgtagtagtagccgggcagttgcacgggcttcttggccatgtagatggtctggaactccaccaggtagtggccgccgtccttcagcttcagggcctggt ggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttcttctgcattacggggccgtcgggggggaagttggtgccgcgcatc ttcaccttgtagatcagcgtgccgtcctgcagggaggagtcctgggtcacggtcaccagaccgccgtcctcgaagttcatcacgcgctcccactggaagccctcggggaaggaca gcttcttgtaatcggggatgtcggcggggtgcttcacgtacgccttggagcg |
| Fig. 2b, Extended Data Fig. 6 | 1mm-A-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccgtcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tcggcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 1mm-B-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccttctgcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgctttacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 2mm-A-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccatcccatagtcttct tctgcgttacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 2mm-B-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccttctgcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgctttacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 4mm-A-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagcggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgtgggggtacaggcgctcggtggtggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttctagatcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 4mm-B-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgccgtccttcggcttcagggcctggtggatctcgcccttcagaacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtagggggggaagttggtgccgcgcatcttcaccttgtagagcagcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 10mm-A-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtggtggccgccgtccttcatcttcagggactggtggatctcgcccttcagcacgccgtcgagggggtacaggcgcgcggtggaggcctcccagcccatagtcttct tcggcattacggggacgtcgggggggaagttggtgctgcgcatcttcacattgtagatcatcgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 10mm-B-tdT | tdTomato | CDS | 195 | 1 | actccaccaggtagtggccgacgtccttcagcttcaggtcctggtggatctcgcccttccgcacgccgtcgcggtggtacaggcgcccggtggaggtctcccagcccatagtcttctt cttcattacggggccgtcggggggtaagttggggccgcgcatcttcaccttgtagatcagtgtgccgtcctgcaggg |
| Fig. 2b, Extended Data Fig. 6 | 20mm-tdT | tdTomato | CDS | 195 | 1 | actccaccaggcagtggccgacgtccttcggcttcaggtcctggcggatctcgcccttccgcacgccgccgcggtggtacaggcgctcagtggaggtctcccagcccatagtcttca tcttcattacggggccgtcgcggggtaagttggggccgcccatcttcacgttgtagatcagtgtgccgacctgcaggg |
| Extended Data Fig. 6 | 1nt-insert in frame | tdTomato | CDS | 196 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtgcgaggcctcccagcccatagtcttc ttctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Extended Data Fig. 6 | 1nt-insert out frame | tdTomato | CDS | 196 | 1 | Actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcagtacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagaatcagcgtgccgtcctgcaggg |
| Extended Data Fig. 6 | 1nt-del in frame | tdTomato | CDS | 194 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacgggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Extended Data Fig. 6 | mm (TGC-TAG) | tdTomato | CDS | 196 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggc |
| Extended Data Fig. 6 | mm (TGT-TAG) | tdTomato | CDS | 255 | 1 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatggtcttct tctgcattacggggccgtcgggggggaagttggtagcgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggaggagtcctgggtcacggtcaccagaccgccgtcctcg aagttcatcacgcgctcccact |
| Fig. 2c | Promotor | Cheta-tdTom | Promotor | 238 | 1 | agttttaaacagagaggaatctttgcagctaatggaccttctaggtctggaaaggagtgggaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccga gaagttagggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggaaaactgggaaagtgaagtcgtgtactggctccgcctttttcccgagggtgggggag aaccgtatat |
| Fig. 2c | Cheta1 | Cheta-tdTom | CDS | 1 | ggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacg gtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattaggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcg gcaccgaccctttggcacagt | |
| Fig. 2c | Cheta2 | Cheta-tdTom | CDS | 1 | ggttctcctgctgtagtcgttgctcaggccggtgaggttgctcaggcggataaggatgacaggacaagtgatgcagccaggttgcatagcgcagccactgcacccggtgtcctgtA gcaaggtagagcaagagggattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagccgcatgtagatttccaggtttgg taggcatagaacatcagcagcaaaatgctgaatcctgctgcaagccactgcaggacatt | |
| Fig. 2c | tdT1 | Cheta-tdTom | CDS | 195 | 1 | Actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2c | tdT2 | Cheta-tdTom | CDS | 228 | 1 | agccctcggggaaggacagcttcttgtaatcggggatgtcggcggggtgcttcacgtacgccttggagccgtacaggaactggggggacaggatgtcccaggcgaagggcagg gggccgcccttggtcaccttcagcttAgcggtctgggtgccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgttcatggagccctccatgcgcacct |
| Fig. 2d | 2TAG | Cheta-tdTom | CDS | 255 | 2 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtagcgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggaggagtcctgggtcacggtcaccagaccgccgtcctcg aagttcatcacgcgctcccact |
| Fig. 2d | 3TAG | Cheta-tdTom | CDS | 255 | 3 | actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtagaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtagcgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggaggagtcctgggtcacggtcaccagaccgccgtcctcg aagttcatcacgcgctcccact |
| Fig. 2g–i | Cheta1-tdT1 | Cheta-tdTom | CDS | 447 | 2 | Ggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctac ggtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattaggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggc ggcaccgaccctttggcacagtActccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtgga ggcctcccagcccatagtcttcttctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcaggg |
| Fig. 2g–i | tdT1-Cheta1 | Cheta-tdTom | CDS-UTR | 447 | 2 | Actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggGgtggttttgcggatatctccgtgaatcaatatgtgctc gtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcg ggccccaaaatgaagagaattaggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagt |
| Fig. 2g–i | Cheta1-Cheta2 | Cheta-tdTom | CDS | 543 | 2 | ggtggttttgcggatatctccgtgaatcaatatgtgctcgtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacg gtggagccatagacgctcaggacgccaaaaccttcgggccccaaaatgaagagaattaggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcg gcaccgaccctttggcacagtggttctcctgctgtagtcgttgctcaggccggtgaggttgctcaggcggataaggatgacaggacaagtgatgcagccaggttgcatagcgcag ccactgcacccggtgtcctgtAgcaaggtagagcaagagggattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagc cgcatgtagatttccaggtttggtaggcatagaacatcagcagcaaaatgctgaatcctgctgcaagccactgcaggacatt |
| Fig. 2g–i | tdT-Cheta1-cheta2 | Cheta-tdTom | CDS | 738 | 3 | Actccaccaggtagtggccgccgtccttcagcttcagggcctggtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggaggcctcccagcccatagtcttct tctgcattacggggccgtcgggggggaagttggtgccgcgcatcttcaccttgtagatcagcgtgccgtcctgcagggggtggttttgcggatatctccgtgaatcaatatgtgctc gtggatcaggacgcgcaggtagtgtcccaacaacccccaacaatttttactcatcagatcaataatcgtgtgacctacggtggagccatagacgctcaggacgccaaaaccttcg ggccccaaaatgaagagaattaggaacataccccagctcacgaaaaacagccatgccatgccggtcacgacctggcggcaccgaccctttggcacagtggttctcctgctgtagt cgttgctcaggccggtgaggttgctcaggcggataaggatgacaggacaagtgatgcagccaggttgcatagcgcagccactgcacccggtgtcctgtAgcaaggtagagcaa gagggattcttaaactcaaaaaagaactcgagaatcaccttaaccatttcaatggcgcacacatagatctcctcccagccgcatgtagatttccaggtttggtaggcatagaacat cagcagcaaaatgctgaatcctgctgcaagccactgcaggacatt |
| Fig.3 | EEF1A1-3’ UTR | Human-EEF1A1 | 3’ UTR | 225 | 1 | cttcagtctccacccactcagtgtggggaaactccatcgcataaaacccctccccccaacctaaagacgacgtactccaaaagctcgagaactaatcgaggtgcctggacggc gcccggtactccgtagagtcacatgaagcgacggctgaggacggaaaggcccttttcctttgtgtgggtgactcacccgcccgctctcccgagcgccgcgtcctccattttg |
| Fig.3 | EEF1A1-5’ UTR | Human-EEF1A1 | 5’ UTR | 249 | 1 | ctcatagggtgtacactagccactacacttatttcttatgtcatggcaaatagtcaactttcactgcccagtcattttaacccacgtttcaacatgcacatcccagtaatttagaaa cattttgtttccaaagattcacttaacattggtttagcaacaggaagctttctatgcaacacaaggactcagtttttggcctgttttagtgacaggcaatcagcaacatgctgcat ttctctccagtgttgt |
| Fig.3 | EEF1A1-intron | Human-EEF1A1 | Intron | 228 | 1 | gtaaggattaagagtcgttacttggttactaaaacacaaactccagcttcaatttccttgtccccagcccttaattagcagtttccactttacaactccaagtccaaagtgatttta gtcactttgggttacagaagcaaccaaaaatcaaacttttataagtcggatcttaactatgaacatccaaatctactcactagcaatacgattacagaagtcaccaaaagc |
| Fig.3 | EEF1A1-Exon3 | Human-EEF1A1 | Exon 3 | 249 | 1 | gttagcacttggctccagcatgttgtcaccattccaaccagaaattggcacaaatgctactgtgtcggggttgtagccaattttcttaatgtaagtgctgacttccttaacaatttc ctcatatctcttctggctgtagggtagctcagtggaatccattttgttaacaccgacaatgagttgtttcacacccagtgtgtaagccagaagggcatgctctcgggtctgcccat tcttggagataccagc |
| Fig.3 | EEF1A1-Exon5 | Human-EEF1A1 | Exon 5 | 249 | 1 | agtgaagccagctgcttccattggtgggtcatttttgctgtcaccagcaacgttgccacgacgaacatccttgacagacacattcttgacattgaagcccacattgtccccagga agagcttcactcaaagcttcatggtgcatttcgacagattttacttccgttgtaacgttgactagagcaaaggtgaccaccataccgggtttgagaacaccagtctccactcggc caacaggaacagtaccaat |
| Fig.3 | EEF1A1-eie | Human-EEF1A1 | Exon 2 | 249 | 1 | gtctgtctcatatcacgaacagcaaagcgacctattaaaaaaaaagttaattattacccaaagtactgttcagttgtatttttcatctttaacacaacttttttacatttaagtagt catccttacccaaaggtggatagtctgagaagctctcaacacacataggcttgccaggaaccatatcaacaatggcagcatcaccagacttcaagaattgagggccatcttcc agctttttaccagaacggc |
| Fig.3 | EEF1A1-CDS | Human-EEF1A1 | CDS | 249 | 1 | gccatcttccagctttttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcatgcaatgtgagccgtgtggcaatccaatacaggggcatagccggcgcttattt ggcctagatggttcaggataatcacctgagcagtgaagccagctgcttccattggtgggtcatttttgctgtcaccagcaacgttgccacgacgaacatccttgacagacacat tcttgacattgaagcccac |
| Fig.3 | Xist-1 | Human-XIST | lncRNA | 234 | 1 | aaaatatggaggacgtgtcaagaagacactaggagaaagtatagaattgaaaaaaatattttatggaatttaggtgatttttttaaagaaatacgccataaagggtgttagg ggactagaaaatgttctagaaagaaccccaagtgcagagagatcttcagtcaggaagcttccagccccgagagagtaagaaatatggctgcagcagcgaattgcagcgctt taagaactgaa |
| Fig.3 | Xist-2 | Human- XIST |
lncRNA | 210 | 1 | caattgggactgagcattttaactgtccaacaaaagacgggttgtctgcgaccccctgctgaatgcaaatggggttcagagtgctgtctaatccaataggtaggattattggca attagaattttactcaatgcaaagagttttaggtggccaacacagtacacaagaaaagatcactactcaaaattgggactgggactacagaagcaa |
| Fig.3 | PCNA-E1 | Human-PCNA | Exon 1 | 219 | 1 | ctggtgaggttcacgcccatggccaggttgcggtcgcagcgggaggtgtcgaagccctcagaccgcagggtgagctgcaccaaagagacgtaggacgagtccatgctctgc aggtttacaccgctggagctaatatcccagcaggcctcgttgatgaggtccttgagtgcctccaacaccttcttgaggatggagccctggaccaggcgcgcctcgaac |
| Fig.3 | ActB-CDS | Human-ActB | CDS | 249 | 1 | CTGGGTGCCAGGGCAGTGATCTCCTTCTGCATCCTGTCGGCAATGCCAGGGTACATGGTGGTGCCGCCAGACAGCACTGTGTTGGCG TACAGGTCTTTGCGGATGTCCACGTCACACTTCATGATGGAGTTGAAGGTAGTTTCGTAGATGCCACAGGACTCCATGCCCAGGAAG GAAGGCTGGAAGAGTGCCTCAGGGCAGCGGAACCGCTCATTGCCAATGGTGATGACCTGGCCGTCAGGCAGCTCG |
| Fig.3 | ActB-2 Exon2 | Human-ActB | Exon 2 | 237 | 1 | gggtcatcttctcgcggttggccttggggttcaggggggcctcggtcagcagcacggggtgctcctcgggagccacacgcagctcattgtagaaggtgtggtgccagattttct ccatgtcgtcccagttggggacgatgccgtgctcgatagggtacttcagggggaggatgcctctcttgctctgggcctcgtcgcccacataggaatccttctgacccatgcccac catcacgc |
| Fig.3 | TP53-1-E3 | Human-TP53 | Exon 3 | 279 | 1 | cgtgcaagtcacagacttggctgtcccagaatgcaagaagcccagacggaaaccgtagctgccctggtaggttttctgggaagggacagaagatggcaggggccaggagg gggctggtgcaggggccgccggtgtaggagctgctAgtgcaggggccacggggggagcagcctctggcattctgggagcttcatctggacctgggtcttcagtgaaccattg ttcaatatcgtccggggacagcatcaaatcatccattgcttgggacggcaaggggga |
| Fig.3 | TP53-2-5UTR | Human-TP53 | 5’ UTR | 240 | 1 | gagatggagtctctctctgtcacctgggctggagcacagtggcatgatctcagctcactgcaacctctaccttccgggttcaagccattctcctgcctcagtctcccgagtagctA ggattacaggcgagtaccaccacacccagctaatttttgtatttttagtagagacagggctttgcatgttggccaggctggtctcgaactccttacttcaggtgatcggcccgcct cagcctcc |
| Fig.3 | HER2-1-E27-1 | Human-HER2 | Exon 27 | 354 | 1 | tcacactggcacgtccagacccaggtactctgggttctctgccgtaggtgtccctttgaaggtgctgggtggagccccccgctctggtgggtcctggtcccagtaatagaggttg tcgaaggctAggctgaaggcaggaggagggtggggctgaggggcagctcctccctggggtgtcaagtactcggggttctccacggcacccccaaaggcaaaaacgtctttg acgaccccattcttccctggggagagagtcttgggcctttccagagtggcaccagcaggtcgggcagcaggcagagggccctctcggggcgaagggggctggggccgaaca tctggctggttcacata |
| Fig.3 | HER2-2-E27-2 | Human-HER2 | Exon 27 | 354 | 2 | tcacactggcacgtccagacccaggtactctgggttctctgccgtaggtgtccctttgaaggtgctgggtggagccccccgctctggtgggtcctggtcccagtaatagaggttg tcgaaggctAggctgaaggcaggaggagggtggggctgaggggcagctcctccctggggtgtcaagtactcggggttctccacggcacccccaaaggcaaaaacgtctttg acgaccccattcttccctAgggagagagtcttgggcctttccagagtggcaccagcaggtcgggcagcaggcagagggccctctcggggcgaagggggctggggccgaac atctggctggttcacata |
| Fig.3 | HER2-3-E3-1 | Human-HER2 | Exon 3 | 273 | 1 | cagtggccctggtcagctctgaataaccaagagaaggtttcaatgacggtgaaggccacctgtgaggcttcgaagctgcagctcccgcaggcctcctggggaggcccctgtg acaggggtggtattgttcagcgggtctccattgtctggcacggccagggcatagttgtcctcaaagagctgggtgcctcgcacaatccgcagcctctgcagtAggacctgcctc acttggttgtgagcgatgagcacgtagccctgcacctcctggatatc |
| Fig.3 | ARC-1-CDS | Human-ARC | CDS | 219 | 1 | agaacacctcgcgccacacgtgcatctcgcgcttggcccagcgctccaggttggcgatggtctcctggcagcggcacaggcaggccttggtggacttcttccagcgctgcgag tcgctcgtAggcacgtagccgtccaggttgctctccagcttcccgaccgaccggtgcagccccttcagctcgcgctccacctgcttggacacctcggccagcaggt |
| Fig.3 | ARC-2-CDS | Human-ARC | CDS | 265 | 1 | cgcaggaaacgcttgagcttgggctgcagggtgcccaccacgtactggatgatctcctcctcgtccgcgtccacgtggagcgtctggtacaggtcccgcttgcgccacaggaa ctAgtccagcggctcgccctgcttctgcggcaggtccagctcgcgctgggtggcctctcgggacagcgtgccctcgctgtactgcaggaactccttcttgaactccacccagttc ttcacggagccctgcttgaactcccaccacttcttgg |
| Fig.3 | ARC-3-CDS | Human-ARC | CDS | 276 | 1 | cttctgctctgggtgtcccatgagcttggtggccgctggcccctgccatccggacacttggttttctggatgtccagagggcctgggtgttgtagcagaatgaggaagctggggt gttctgtgatggcctgggagtgtggagggtggggtccaggccggaaagacagacggagggagggtgatgggtAgagccctgcaggggggagggctctttgccgctgggtg cggtgctcacatctggatgacacgagtgtgtgcgagtccgcccggagctcg |
| Extended Data Fig. 9 | Ctip2-ses1 | Mouse-Ctip2 | Exon 4 | 384 | 1 | gaagcgcaggctgccgttctcagaggaatgttccgacgatgtggcgaaaggcgactggcgcgcatccgtgaagcccaggaatgggtccttcatgaagtggcgcgatgctgc gtagcccacgagccactgcgagtacacgttctcagatgggatgagggcggcaggtagcagctccaggtctttctccaccttgatgcgcttggccgcgtgcagcgcgggaccac cgagcccagggctgggcagcggtgctggcttgcgtgggaagagagctggaaagggctctgcgcctggcgcgaaggccccgccgcgcccgttcactgcacccggtgcacccg cgtccccacagccaacagctccggcatcacccgtgtcgcctgcacgctt |
| Extended Data Fig. 9 | Ctip2-ses2 | Mouse-Ctip2 | Exon 3 | 252 | 1 | caccgcagcggctctgttaccggacaactcacactggcatccaaagggagcctccatctgaccctcaccctgagtcccgtcgcccgagatggggcgtgcaccacagcaaggc agcgggaaacagggtaggagaacgcccagggcacgcagaggggaagtaatcacggaggtgggagggtgggaagaggaggcagctatgggggccatcgatggcagctg ggcaggcctgcacggccctggagaaaaaacaat |
| Fig. 4, Extended Data Fig. 9 | Ctip2-ses3 | Mouse-Ctip2 | Intron 3 | 288 | 1 | acattaagaatcggtctgttcccaggagttctgtctccaaggtcacaccatgtctgggaaggaaaactgttccttcacgcaaatgctcgcagcaacttcccagtaccctttatcct tgtgtagccacctgcctcgctctctacactgcagagactgatgaagcactgtgagccagggccatggagcaggagaagggctgctttcacggaaccacagtcagcaagacat cggggattacgtgatttccagcgagggttcaagtatcgagtccataagaaaggtgagtcc |
| Extended Data Fig. 9 | Ctip2-ses4 | Mouse-Ctip2 | Exon 4 | 207 | 2 | ttccgacgatgtggcgaaaggcgactggcgcgcatccgtgaagcccaggaatgggtccttcatgaagtggcgcgatgctgcgtagcccacgagccactgcgagtacacgttc tcagataggatgagggcggcaggtagcagctccaggtctttctccaccttgatgcgcttggccgcgtgcagcgcgggaccaccgagcccagggct |
| Extended Data Fig. 9 | Ctip2-ses5 | Mouse-Ctip2 | Exon 2 | 345 | 2 | tgcaatgttctcctgcttgggacagatgcctttcgtgggggacaggaggtggtcatcttcatcaggggtgacctggatcccgatctccactagctcagatactctcctgagctca gagcgagaggagggaggtagactgctcttgtccaggaccttgtcgtagcaggggcccaggcctccacactgtttcttcttgtgctctataaaaaccaggatgtcccccagcgg gaagttcatctgacactggccacaggtgaggagatcagggtcggggcctcccaccatcagccccaggctgctaggctcctctatctccagaccctcgtcttcctcgaggatggt agc |
| Extended Data Fig. 9 | Ctip2-ses6 | Mouse-Ctip2 | Mix: Exon 4/2 | 411 | 3 | ttccgacgatgtggcgaaaggcgactggcgcgcatccgtgaagcccaggaatgggtccttcatgaagtggcgcgatgctgcgtagcccacgagccactgcgagtacacgttc tcagataggatgagggcggcaggtagcagctccaggtctttctccaccttgatgcgcttggccgcgtgcagcgcgggaccaccgagcccagggcttttgtggtcatcttcatca ggggtgacctggatcccgatctccactggctcagatactctcctgagctcagagcgagaggagggaggtagactgctcttgtccaggaccttgtcgtagcaggggcccaggcc tccacactgtttcttcttgtgctctataaaaaccaggatgtcccccagcgggaagttcatctgacactggcc |
| Extended Data Fig. 9 | Ctip2-ses7 | Mouse-Ctip2 | Intron 1 | 300 | 2 | agtcctccagaaatatacagatgcattgtgcccgcccggcacacaggaaaggaagaacaccgactgagacataggagaagggtgtcaaaatattgtttggtcagaaaaga aacaaaaagggacaaaggaaataaactgagaagcaaaacaaaatagggataggcggggggcaaactgaaattgaaattgagaattcaaactaagcagggaggggtgc tatccctgggagggatccactgcccgcctcgagcaggcctggcaaggcctctgctccttacagcctttgataaaaataataaa |
| Fig. 4, Extended Data Fig. 9 | Ctip2-ses8 | Mouse-Ctip2 | Exon 4 | 765 | 2 | gagcttgctcgcctgcgagcacgcatggtcgcacagctggcacttgtagggcttctcgcccgtgtggctgcgccggtgcacgatgagattgctctggaacttgaaggtcttgcc gcagaactcacaggacttgctcttggcaggcggctgcggtggcggtgtgcccgcaggcatgggtggcagcggtggcgtgctgaggaacggggacttgggactgggctggaa agggttcagcagccggtgcatagggttgccacggcctggggacacgggcggcggcgtggagctgttgccggccagttctcgcagccgccgggagaagtccatggcaggag agtctatggccatggggttcaggcgcatgactcggtcgaaggcactggggtgctgggccacgagccccatctcctctgcactgaggcggtgtgggtccaagtgatggcgtagc ggtgggctgaagagcggtggcgtacctagcaagcggccctcaccgaagccagggtggtcccgcaggatggggcccgtcatgcgcagcaggttgaaaggattgctgtccccc aggaaattcatgagtggggactgcgccacggtctccggcccgagcggtggcgggatggtgagcctgggcgtgagcgaggtgctggccggcccaggctccaggtagattcgg aagccatgtgtgttctgtgcgtgctgcagcaggaaccaggcgctgttgaagggctgcttgcatgttgtgcaaatgtagctggaaggctcatcttt |
| Extended Data Fig. 9 | Fezf2-ses1 | Mouse-Fezf2 | Exon1 | 189 | 1 | cgcagccaggctggccagtttggcgttctccagcagaaaaagcttggggtgagcagccagggaagtgggggcctgtgcgttgaggaggccggataggaaaaggtggcctc cgaggagctccgaaggtgggtaagcggtggagtccaggtagttgaagtagtagagagagccgctggcaggcagccccac |
| Extended Data Fig. 9 | Fezf2-ses2 | Mouse-Fezf2 | Exon1 | 204 | 2 | gaacttgtccgcagccaggctggccagtttggcgttctccagcagaaaaagcttggggtgagcagccagggaagtgggggcctgtgcgttgaggaggccggataggaaaa ggtggcctccgaggagctccgaaggtaggtaagcggtggagtccaggtagttgaagtagtagagagagccgctggcaggcagccccacagcctg |
| Extended Data Fig. 9 | Fezf2-ses3 | Mouse-Fezf2 | 5’ UTR | 372 | 2 | gcacctagtcggcccggtcacccatttgcattcaaaggaacagaagcagaacaaagtgcacccaagggtaccaaattaccgcccattaacccggcgcgcaaaggaaccca ccagctattgccctaggactatgaaagggggaagaagggggaggctgtacaaaggaaaggagaaggggtagaagaaaggagtctcaaattaacactcccagcccttccc tagacccaggccttcgccacgcgtgctgggaactgggcactgaaagagcaaagtgaaagagggccggaagaaccacctcgcttttacctctttcccccaccgccaaggaga tgcgttccgagccatgcagcgtgtctcttctgtcacattttg |
| Extended Data Fig. 9 | Fezf2-ses4 | Mouse-Fezf2 | Exon1 | 753 | 2 | cttgccgcacacttcgcaggtgaagtttttgggtttgctgtcagtagagccccccgggagtttgctgtggctcttgactccccctcgttcagctgtcaaggccgagttctccttcag cacctgctccagtggcgcatgcaagcgctccttatggggataggaagctgggtgggggaacttgtccgcagccaggctggccagtttggcgttctccagcagaaaaagcttg gggtgagcagccagggaagtgggggcctgtgcgttgaggaggccggataggaaaaggtggcctccgaggagctccgaaggtaggtaagcggtggagtccaggtagttga agtagtagagagagccgctggcaggcagccccacagcctggttgatgacctgcggtttgatgaccctgccggcgggcagcgcagaaggcgcgaggcccagttcggccttgc agcacacgccacagttggttttgcacaagccgctggcgccgcacactggggccccaccgccgctgcctcctcctccaccgccgcccgcccggaggctgctcttccagaactcc gagtaactgagcagcgtcttggacggcacctcgtagccgagaggctggagagggatcatacagggcagcggcgagcagaggttgagcagtttcttgctctggctgctgtctg cctcgaacgcagcaggccggggctcgaaaggcgctcggggctcggacgtcttggccatgatgcgctcgatagagaaagc |
| Extended Data Fig. 12 | Satb2-sesRNA | Mouse-SatB2 | CDS | 708 | 2 | ttatctctggtcggtttcggccggtgcagccttgctcttgtcggcattctcttcctcggcctccaccttgtacatctcctccgagccttcctcactgtcattctcctcggactcggtca gcagctcctcgtccttatactctgccacgtccacggcggagcccaggtgctccttcagcttgccatggtgcttcacgtggtacctctggttctggaagaacttgatgatggtgtgt ttggggagatccagctgggcggagagtgtgtggatggcttcctggtcgggatagaggcctacatcatggatgaagctttgaaggatgcccagggcttccaaagagatctttg tgcgagaccgaggctttttggcacagctgtcttctgttagaggaggcggtaggggtgcttcttctctgggaggggagctctccttagttggctgggactgctgtcgatgaagga cctgcacgggctcaggtgggagctggaccacaggctgcatgcgttcactgtggtgaggtcgagattcttcctcatagatcacatcccgctcaggttggggaagattcaggaaa cggcggatggtgcagagattctcccaaagggtgcggttttctgggctggggttctccttccaacgaagcagttcgcaaagccagccctgacttttgtttgcagccactttggca aacagggcttgagacacctt |
| Extended Data Fig. 12 | plxnD1-sesRNA | Mouse-PlxnD1 | exon1 | 294 | 1 | gcggggcaggcagatgcgcgccagcaggctgcgcgcctggctgtccttgtcgcccgcacgcgcctcgctgttgagcgccaggtacgcgtagggctgcgcgctgtgcggtagc accgccgggtgcaagaaggcacgcacgaagcccagcttgtgctgctccttggcgccctgcttgatcttcaggatgttatcgtccgacgggttaaggtcgaaggtgaagagctt ggccaagtctccacgcgcgtccagggagcggatagcgatctcgggcgtgttctcgaagcggtggtcttc |
| Extended Data Fig. 12 | Rorb-sesRNA | Mouse-Rorb | exon4 | 393 | 1 | tacgatctcagacattgttatcccgggagcgaactgcccgttgttgaaagagctataggtaaacaagttgggtacagatgtgaggtcatagataggttcttgctttatctgtttg atcccagtcatgtccagctcctgactgatcgggagacggctgaccggaatctatgctgtaataaccttcggacttaggcaggtcaatgcgtgcccgttggcgtatgtgccgccg gtctcggtgttcaggttgctgaggccattgctaatgctgctgctgtacaccctggcgagggcctccgcctccccactctgctgctgccgctgctcctgcagcctttgctgaggctt ctgcacctcagcatacaggctgtcccgctgcttcttggacatcctccc |
| Extended Data Fig. 12 | vGAT-sesRNA | Mouse-vGAT | exon1 | 300 | 2 | cctggtccttggagccagagggtggcagaggagcgccgccgcgctgataatgaatgtctccctcgacgggagcttctgcgccctcgtctccgcagggctcgccttccgatttca ggatgtccatctgcaggccctggcgatgctcaaagtcgagatcgtcgcagtgcgcgaagcccaccgcttcctcatcggtagccgcctgaaaccccatcctagcaaacatgccg ctcaccttggcctgggacttgttggacacggaggtggccacattggtcagcttgctgcggagcagggtggcca |
| Extended Data Fig. 15 | Tle4-sesRNA | Rat-Tle4 | exon15 | 194 | 2 | atgagaagcagaccttggagtcggggctgttggccagagcatagcaggcaggggctgaggacgtcagctctgccttgttgcgtggggttggagctgccaggtcccagatag acagtgtactagcttcccctccaacaattaaggtgcggccatcagggagcaatctgcaggagcggatgtagttatccctgt |
| Extended Data Fig. 15 | vGAT-sesRNA | Rat-vGAT | exon2 | 255 | 1 | ggccacatcgaagaagacctggtgccacagcagcttgcgccagagaagacgcaagtggaagaggctgggcagcaggaagcagaggccggctcccgtgaggctgcccgtg aggcccatgagcagcgcgaagtgtagcacgtagatggccatgagcagcgtgaagaccaccagcgcgcagcgcagcgtcagcccccaggacttaaggcgaccgtcgccacc gtagcaggcggggaagaaggcacgactgccttcctg |
| Fig. 5, Extended Data Fig. 16 | FOXP2-1-sesRNA | Human-FOXP2-1 | CDS | 264 | 1 | ggtaagctctgtggggatgtctccaacatattcttcgacatggtgacactagacaccagatttagaggtttgggagatggtttgggctctgagggtcgcatgtgcaagtaggtc atcattgctggaagacgttcgcgttctttagaaagctgtatttctaactgttgcaccacctgcatttgcactcgacacggagcagtgcttcggtcatccaatgcgtgttcattgtta aggtgctttaaaaactgtccaaaatcttcacaa |
| Fig. 5, Extended Data Fig. 16 | FOXP2-2-sesRNA | Human-FOXP2-2 | CDS | 279 | 2 | ctggccatttgcaaactccatggccatagagagtgtgagaggccccagtctcctcatgtgacgagctgtctcgtcttgcacttagaactgaagactgtccattcactatagaat gatgagttattggtggtggtgctttagaagtgttggaggaggaagtcgaggaggaattgtgagtggtgcggtctagccctccatgttgaatgccattgtcttccatactgtgaa ctccagtcacttctttccataactgctgaatctcagcaggacttaagccag |
| Fig. 5, Extended Data Fig. 16 | VGAT-sesRNA | Human-VGAT | Exon2 | 202 | 1 | aggacttcaggcgcccgtcgccgctgtagcaggccgggaaaaaggcgcggctgccttcctggaagagcgacttctccagcacctcgacagcggcaaagaatagcagagga taggacaacagcgccttggccaccagaaagatgttgaccacggcgcggatggagccgggcaggttatccgtgatgacctccttggtctcgtc |