Table 1.
Intestinal microbiota adhesion proteins that bind to mucin-type glycan epitopes
| Commensal bacteria | Proteina | Target substrateb | Glycan epitopec |
|---|---|---|---|
| Bifidobacterium longum BBMN68114 | FimM | Human epithelial cell lines, PGM | |
| Bifidobacterium bifidum ATCC 15696115 | Sialidase (SiaBb2) | PCM, mice colonic tissue sections | α2,6-linked sialic acid, Blood group A |
| Bifidobacterium bifidum JCM 1254116 | CBM32 domain of sulfoglycosidase (BbhII) | In vitro assays, PGM, PCM | N-acetylglucosamine-6-sulfate |
| Bifidobacterium bifidum A8117 | Transaldolase (Tal) | Human intestinal epithelial cell lines, PGM | |
| Bifidobacterium longum NCC2705118 | GroEL, Transaldolase (Tal) | PGM | |
| Bifidobacterium longum subsp.longum119 | Fimbrial protein | PCM | |
| Bifidobacterium longum subsp. Infantis120 | Blon_0343 and Blon_2202 (F1SBPs) | Mammalian glycan array | Blood group type H, 2’-fucosyllactose |
| Blon_0883 (F1SBPs) | Mammalian glycan array | Type 1 glycans, Lewis a, Blood group H type 1 | |
| Blon_2177 (F1SBPs) | Mammalian glycan array | Type 1 glycans | |
| Escherichia coli Nissle 1917 (EcN)121 | flagellum | Human epithelial cell lines, porcine MUC2, human colonic mucus | |
| Lactobacillus fermentum 104R122 | Mucin-binding protein (MucBP) | Porcine small intestinal mucus, PGM | |
| Lactococcus lactis (TIL448)123 | Pilin and mucus-binding protein | PGM | Neutral oligosaccharides |
| Lactobacillus rhamnosus 124 | Pilin | Colonic mucus from colorectal cancer patients | |
| Lactobacillus johnsonii NCC533 (La1)125, 126 | EF-Tu and GroEL | Human intestinal epithelial cell lines, HCM | |
| Lactobacillus paracasei subsp. paracase127 | EF-Tu and GroEL | Micro Arrays | α2,3-linked sialic acid, α1,2-linked fucose |
| Lactobacillus reuteri 1063128 | Mub | Porcine gastric and colonic mucus | |
| Ruminococcus gnavus ATCC 29149129 | CBM40 domain of trans-sialidase (RgNanH) | In vitro glycan arrays, mouse colonic tissue sections and binding assays | α2,3/2,6-sialyllactose |
a
Only proteins with described binding to mucins or mucus were included in the table.
b
CBM, carbohydrate binding module; PGM, porcine gastric mucin; PCM, porcine colonic mucin; HCM, human colonic mucus; F1SBPs, Family 1 of solute binding proteins; MUC2, Mucin 2.
c
When reported, the described target glycans are shown on the column on the Glycan epitope column.