Table 1.
Table of summary statistics
| Figure | Parameter | Type of test | Sample size | Statistical data |
|---|---|---|---|---|
| 3A | [35S]GTPγS binding | Nonlinear curve fit | WT male = 7 Cre/+ male = 7 |
EC50 WT = 1.33 × 10–7 M for WT and 1.268 × 10−7 for Cre/+ R2 = 0.7756 for WT and 0.7597 for Cre/+ |
| 3B | P-ERK levels normalized to T-ERK | Unpaired t test Two tailed |
WT female = 4, Cre/+ female = 3, Cre/+ male = 2 |
t = 0.68, df = 10, p = 0.51 |
| 3C | P-JNK levels to T-JNK | Unpaired t test, two-tailed | WT female = 4, Cre/+ female = 3, Cre/+ male = 2 |
t = 0.498. df =7, p = 0.6335 |
| 3D | P-p38 levels normalized to total p38 | Unpaired t test, two-tailed | WT female = 4, Cre/+ female = 3, Cre/+ male = 2 |
t = 0.043, df =10. p =0. 97 |
| 4A | Elevated plus maze, percentage open arm entries | Two-way ANOVA | WT male = 7, WT female = 12; Cre/+ male = 8, Cre/+ female = 7 |
Fgenotype (1,30) = 0.21, p = 0.66 Fsex (1,30) = 4.2, p = 0.049 Fgenotype × sex (1,30) = 0.32, p = 0.57 |
| 4B | Elevated plus maze, percentage open arm time | Two-way ANOVA | WT male = 7, WT female = 12; Cre/+ male = 8, Cre/+ female = 7 |
Fgenotype (1,30) = 0.74, p = 0.4 Fsex (1,30) = 0.99, p = 0.33 Fgenotype × sex (1,30) = 0.46, p = 0.50 |
| 4C | Percentage time in center, open field | Two-way ANOVA | WT male = 8, WT female = 11; Cre/+ male = 9, Cre/+ female = 7 |
Fgenotype (1,31) = 1.97, p = 0.17 Fsex (1,31) = 4.4, p = 0.04 Fgenotype × sex (1,31) = 0.00003, p = 0.99 |
| 4D | Mean withdrawal threshold, electronic von Frey | Two-way ANOVA | WT male = 8, WT female = 10; Cre/+ male = 8, Cre/+ female = 7 |
Fgenotype (1,29) = 0.18, p = 0.68 Fsex (1,29) = 9.82, p = 0.0039 Fgenotype × sex (1,29) = 3.78, p = 0.06 |
| 4E | Response latency, hot plate | Two-way ANOVA | WT male = 8, WT female = 10; Cre/+ male = 8, Cre/+ female = 7 |
Fgenotype (1,29) = 1.28, p = 0.27 Fsex (1,29) = 3.76, p = 0.06 Fgenotype × sex (1,29) = 2.58, p = 0.12 |
| 4F | Response latency, cold plate |
Two-way ANOVA | WT male = 8, WT female = 9; Cre/+ male = 9, Cre/+ female = 7 |
Fgenotype (1,29) = 0.09, p = 0.77 Fsex (1,29) = 1.7, p = 0.2 Fgenotype × sex (1,29) = 1.94, p = 0.17 |
| 5B | Number of action potentials | Paired t test, two-tailed | Baseline = 7, CNO = 7 | t = 3.08, df = 9, p = 0.022 |
| 5C | Resting membrane potential | Paired t test, two-tailed | Baseline = 7, CNO = 7 | t = 3.757, df = 9, p = 0.0094 |
| 5E | c-Fos counts | Unpaired t test, two-tailed | Veh = 3, CNO = 4 | t = 4.995, df = 5, p = 0.0041 |
| 7A | Elevated plus maze, percentage open arm entries | Two-way ANOVA | mCherry male = 8, mCherry female = 10; hM3DQ male = 8, hM3DQ female = 9 |
Fvirus (1,31) = 6.64, p = 0.015 Fsex (1,31) = 2.51, p = 0.12 Fvirus × sex (1,31) = 7.49, p = 0.01 |
| 7B | Elevated plus maze, percentage open arm time | Two-way ANOVA | mCherry male = 8, mCherry female = 10; hM3DQ male = 8, hM3DQ female = 9 |
Fvirus (1,31) = 9.4, p = 0.005 Fsex (1,31) = 5.07, p = 0.032 Fvirus × sex (1,31) = 12.22, p = 0.001 |
| 7C | Percentage time in center, open field | Two-way ANOVA | mCherry male = 9, mCherry female = 11; hM3DQ male = 9, hM3DQ female = 9 |
Fvirus (1,34) = 1.12, p = 0.29 Fsex (1,34) = 0.03, p = 0.87 Fvirus × sex (1,34) = 0.48, p = 0.49 |
| 7D | Sociability index | Two-way ANOVA | mCherry male = 9, mCherry female = 11; hM3DQ male = 9, hM3DQ female = 10 |
Fvirus (1,35) = 0.48, p = 0.83 Fsex (1,35) = 0.01, p = 0.92 Fvirus × sex (1,35) = 6.76, p = 0.01 |
| 8A | U-50488 CPA, difference score | Two-way ANOVA | mCherry male = 9, mCherry female = 10; hM3DQ male = 8, hM3DQ female = 11 |
Fvirus (1,34) = 1.656, p = 0.21 Fsex (1,34) = 0.016, p = 0.89 Fvirus × sex (1,34) = 4.67, p = 0.037 |
| 8B | CNO CPP, difference score | Two-way ANOVA | mCherry male = 5, mCherry female = 7; hM3DQ male = 6, hM3DQ female = 7 |
Fvirus (1,21) = 0.0004, p = 0.98 Fsex (1,21) = 0.85 p = 0.37 Fvirus × sex (1,21) = 1.005, p = 0.3276 |
| Extended Data 3-1A | Relative Oprk1expression, whole brain | Unpaired t test, two-tailed | WT female = 5 Cre/+ female = 5 |
t = 7.43, df = 8, p < 0.0001 |
| Extended Data 3-1B | Relative Oprk1expression, NAc | Unpaired t test, two-tailed | WT male = 4 WT female = 2 Cre/+ male 3 = Cre/+ female = 5 |
t = 2.934, df = 12, p = 0.013 |
| Extended Data 3-2A | Maximal G-protein stimulation | Unpaired t test, two-tailed | WT male = 7 Cre/+ male = 7 |
t = 0.12, df = 12, p = 0.9 |
| Extended Data 3-2B | EC50 | Unpaired t test, two-tailed | WT male = 7 Cre/+ male = 7 |
t = 0.07, df = 12, p = 0.95 |
| Extended Data 3-2C | Basal [35S]GTPγS binding | Unpaired t test, two-tailed | WT male = 7 Cre/+ male = 7 |
t = 0.22, df = 12, p = 0.83 |
| Extended Data 3-3A | Amygdala p-ERK/T-ERK | Unpaired t test, two-tailed | WT female = 5, Cre/+ male = 2 male, Cre/+ female = 2 | t = 0.712, df = 7, p = 0.499 |
| Extended Data 3-3B | Amygdala p-JNK/T-JNK | Unpaired t test, two-tailed | WT female = 5, Cre/+ male = 2 male, Cre/+ female = 2 | t = 0.715, df = 7, p = 0.498 |
| Extended Data 3-3C | Amygdala P-p38/T-p38 | Unpaired t test, two-tailed | WT female = 5, Cre/+ male = 2 male, Cre/+ female = 2 | t = 1.922, df = 6, p = 0.1030 |
| Extended Data 4-1 | Number of closed arm entries, WT vs Oprk1-Cre | Two-way ANOVA | mCherry male = 7, mCherry female = 12; hM3DQ male = 8, hM3DQ female = 7 |
Fgenotype (1,30) = 1.335, p = 0.26 Fsex (1,30) = 0.76, p = 0.39 Fgenotype × sex (1,30) = 0.66, p = 0.43 |
| Extended Data 7-1 | Number of closed arm entries, mCherry vs hM3DQ | Two-way ANOVA | mCherry male = 8, mCherry female = 10; hM3DQ male = 8, hM3DQ female = 9 |
Fvirus (1,31) = 0.02, p = 0.88 Fsex (1,31) = 0.43, p = 0.52 Fvirus × sex (1,31) = 0.62, p = 0.43 |
| Extended Data 8-1 | Difference score percentage (Post-Pre) | Unpaired t test | WT male = 8–10 | t = 2.322, df =18, p = 0.032 |
P, Phosphorylated; T, total.