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. 2023 Jun 30;14:1185517. doi: 10.3389/fimmu.2023.1185517

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Copyright © 2023 Martín-Adrados, Wculek, Fernández-Bravo, Torres-Ruiz, Valle-Noguera, Gomez-Sánchez, Hernández-Walias, Ferreira, Corraliza, Sancho, Esteban, Rodriguez-Perales, Cruz-Adalia, Nakaya, Salas, Bernardo, Campos-Martín, Martínez-Zamorano, Muñoz-López, Gómez del Moral, Cubero, Blumberg and Martínez-Naves

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Changes in Caco-2 gene expression by flagellin (Fl), thapsigargin (TG) or the combination of both treatments (TGFl). Effect of indicated (Fl, TG or TGFl) treatments compared to non-treated cells (Control), on the expression of ER stress-related (HSPA5), chemokines and cytokines (CXCL8, CXCL2), epithelial reparation (SPRR2A, MMP3, MMP7), NKG2D ligands (ULBP1, ULBP2, ULBP3), cellular proliferation and differentiation (DUSP6, ANXA1) and mitochondrial (HMGCS2) genes. Caco-2 cells were incubated with flagellin 1 μg/mL for 2 hours, with 1 μM TG for 5 hours or with TG for 5 hours and Fl for additional 2 hours. The relative mRNA abundance of the indicated genes was determined by qPCR using GAPDH as the housekeeping gene. Data from three independent experiments are shown, represented as the mean ± SEM of individual groups compared with the control group. Unpaired t test was used. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ns: not significant.