Fig 2. Inhibitors of CbMip affect intracellular replication of C. burnetii.

(A and B) Intracellular replication of C. burnetii NMII in the presence of CbMip inhibitor SF235 (purple square), AN296 (blue triangle) or vehicle control (black circle). (A) THP-1 cells with 50 μM of inhibitor (n ═ 6) and (B) HeLa cells with 100 μM of inhibitor (n ═ 3). Error bars represent standard error of the mean. *, p < 0.05: **, p < 0.01; ****, p < 0.0001. p values were determined using two-way ANOVA, followed by Dunnett’s multiple comparison post-test. (C) Representative confocal immunofluorescence (IF) images at 3 and 5 days post-infection (DPI) for HeLa cells. Cells were stained with anti-LAMP1 (green), anti-Coxiella (red), and Hoechst 33258 (blue). Scale bar ═ 20 μm.