Table 1. Demographic and imaging data.

	Pilocytic astrocytoma	Diffuse midline glioma, H3K27-altered	H3.3	H3.1	p-value (PA vs. DMG)	p-value (H3.3 vs. H3.1)	p-value (PA vs. H3.3)	p-value (PA vs. H3.1)
	n = 23	n = 40	n = 35	n = 5
Age (median years [range])	13 [1–71]	8.5 [1–35]	11 [1–35]	7 [4–9]	0.74	0.16	0.96	0.23
Sex (M:F)	10:13	21:19	20:15	1:4	0.60	0.17	0.42	0.62
Site
Brainstem	11	20	16	4
Thalamus	3	18	17	1
**Other	9	2	2	0
DSC-MRI	n = 14	n = 16	n = 15	n = 1
nrCBV (median [range])	1.17 [0.47–2.85]	1.66 [0.74–4.86]	1.67 [0.74–4.86]	1.76	0.058		0.063
nrCBF (median [range])	1.19 [0.78–3.00]	1.87 [0.91–11.2]	1.87 [0.91–11.2]	1.56	0.028		0.023
PSR (median [range])	1.23 [0.46–3.52]	0.97 [0.55–2.78]	1.00 [0.55–2.78]	0.78	0.13		0.172
DWI	n = 23	n = 40	n = 35	n = 5
nADCmean (median [range])	1.96 [1.43–3.5]	1.48 [1.11–2.76]	1.46 [1.11–2.76]	1.80 [1.59–2.35]	0.00075 *	0.10	0.00020 *	0.68

* Indicates a statistically significant value.

DMG = diffuse midline glioma, H3K27-altered; DSC = dynamic susceptibility contrast; DSC-MRI = dynamic susceptibility contrast magnetic resonance imaging; DWI = diffusion-weighted imaging; nADCmean = normalized mean apparent diffusion coefficient; nrCBF = normalized relative cerebral blood flow; nrCBV = normalized relative cerebral blood volume; PA = pilocytic astrocytoma; PSR = percentage signal recovery.

** Other: cerebellum (n = 9) in PA; septum pellucidum (n = 1) and basal ganglia (n = 1) in DMG (H3.3)