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. 2023 Jul 14;18(7):e0287712. doi: 10.1371/journal.pone.0287712

Fig 2. Absolute quantification of the serially diluted recombinant plasmid.

Fig 2

A representative 1D amplitude plot of ddPCR reactions (A) and amplification curve of qPCR (B). ddPCR threshold is shown by a pink line that divides the results as positive (above the threshold) and negative droplets (under the threshold). The vertical yellow line divides the ddPCR reactions with different diluted targets. A blue-dark line with the position shows qPCR threshold is in the middle of the logarithmic phase of the amplification curve.