Table 1. Target genes for PCR amplification, primers and their characteristics.

PCR reaction mix	Strains	Target genes	Primer sequence	Amplicon size (bp)	Ref.
M-1	EPEC/ STEC	eae	F: 5’-CTGAACGGCGATTACGCGAA-3’ R: 5’-CGAGACGATACGATCCAG-3’	917	[39]
	EPEC (typical)	bfp	F: 5’-AATGGTGCTTGCGCTTGCTGC-3’ R: 5’-GCCGCTTTATCCAACCTGGTA-3’	326
	STEC/ EHEC	stx	F:5′-GAGCGAAATAATTTATATGTG-3′ R: 5′-TGATGATGGCAATTCAGTAT-3′	518
M-2	ETEC	lt	F: 5’-GCACACGGAGCTCCTCAGTC-3’ R: 5’-TCCTTCATCCTTTCAATGGCTTT-3’	218
		st	F: 5’-GCTAAACCAGTAGAG(C)TCTTCAAAA-3’ R: 5’-CCCGGTACAG(A)GCAGGATTACAACA-3’	147
	EIEC	virF	F: 5’-AGCTCAGGCAATGAAACTTTGAC-3’ R: 5’-TGGGCTTGATATTCCGATAAGTC-3’	618
		ipaH	F: 5’-CTCGGCACGTTTTAATAGTCTGG-3’ R: 5’-GTGGAGAGCTGAAGTTTCTCTGC-3’	993
M-3	EAEC	aggR	F: 5’-GTATACACAAAAGAAGGAAGC-3’ R: 5’-ACAGAATCGTCAGCATCAGC-3’	254
		astA	F:5’-CCATCAACACAGTATATCCGA-3’ R: 5’-GGTCGCGAGTGACGGCTTTGT-3’	111	[40]
	DAEC	daaF	F: 5’-GAACGTTGGTTAATGTGGGGTAA-3’ R: 5’-TATTCACCGGTCGGTTATCAGT-3’	542	[39]

bp, base pair; PCR = Polymerase Chain Reaction; EPEC, Enteropathogenic Escherichia coli; ETEC, Enterotoxigenic Escherichia coli; EIEC, Enteroinvasive Escherichia coli; EAEC, Enteroaggregative Escherichia coli; STEC, Shiga-toxin producing Escherichia coli; DEAE, Diffusely adherent Escherichia coli