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. 2023 Jul 3;19(7):e1010827. doi: 10.1371/journal.pgen.1010827

Fig 4. Wolframin acts in Dop2R neurons to maintain sleep via Dop2R signaling.

Fig 4

(A) Daily sleep duration of flies with wfs1 knocked down in dopaminergic neurons, dopamine receptor neurons and Goα+ cells. (B and C) The sleep profile of flies with wfs1 knocked down in Dop2R neurons (B) or Goα+ cells (C). (D) Daily sleep duration of wfs1 mutant, Dop2R neuron rescue and control flies. (E) Daily sleep duration of wfs1 mutant flies with pan-neuronal knock down of Dop2R. Gray shade indicates the dark period. For comparison between RNAi or over-expression flies vs. UAS/GAL4 controls, one-way ANOVA was used: compared to GAL4 control, ##P < 0.01, ###P < 0.001; compared to UAS control, *P < 0.05, **P < 0.01, ***P < 0.001. For comparison between mutant vs. control, Mann-Whitney test or Student’s t-test was used: *P < 0.05, ***P < 0.001. For comparison between wfs1 rescue vs. controls, one-way ANOVA was used: ***P < 0.001. For comparison between mutant expressing RNAi vs. controls, one-way ANOVA was used: *P < 0.05, ***P < 0.001. n = 13–94. Error bars represent SEM. eGAL4, elavGAL4; D2R, Dop2R; G4, GAL4; U, UAS; M, wfs1MI14041; ZT, Zeitgeber Time.