(A-C) The sleep profile (A), daily sleep duration (B), and wake activity (C) of TrpA1 expressing flies and controls under baseline temperature (19°C) and activation temperature (25.4°C). (D-F) The sleep profile (D), daily sleep duration (E), and wake activity (F) of flies expressing NachBac in Dop2R neurons and controls under baseline temperature (19°C) and activation temperature (28°C). (G-I) The sleep profile (G), daily sleep duration (H), and wake activity (I) of flies expressing NachBac in Goα+ cells and controls under baseline temperature (19°C) and activation temperature (27°C). Gray shade indicates the dark period. One-way ANOVA: *P < 0.05, **P < 0.01, ***P < 0.001. n = 23–80. Error bars represent SEM. D2RG4, Dop2RGAL4; G4, GAL4; U, UAS; ZT, Zeitgeber Time.