Fig. 1. Lrp1-depleted MEFs are resistant to RVFV infection.
(A) Schematics showing the generation of mouse embryonic fibroblasts (MEFs) from embryonic day (E) 14.5 embryos obtained by timed mating of Lrp1f/+ mice. dpi, days postinfection. (B) Western blot of Lrp1f/f MEFs infected with adenovirus expressing an empty adenovirus vector (Ad) and the cre recombinase enzyme (Adcre). The blot was probed for lipoprotein receptor–related protein 1 (Lrp1) and β-tubulin. Lrp1-sufficient and Lrp1-deficient MEFs were infected with Rift Valley fever virus (RVFV) MP12GFP at a multiplicity of infection (MOI) of 2 for 16 hours. (C) Fluorescence images show green fluorescent protein (GFP), DAPI (4′,6-diamidino-2-phenylindole), and merged panels. Images were taken at ×20 magnification (scale bars, 200 μm). (D and E) Representative flow cytometry histograms and corresponding analysis of flow cytometry histogram data from MEF infections. Experiment was performed five times in duplicates. Student’s t test was used to determine significance; ****P < 0.0001.