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. 2023 Jul 14;9(28):eadf4766. doi: 10.1126/sciadv.adf4766

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Fig. 1. — (A) Immunoblot analysis of indicated proteins from HEK293T cells transiently transfected with indicated FLAG-tagged RIT1 constructs or an EV control and treated with 10 μM LY3009120 (RAFi) or DMSO vehicle control for 1 hour. One of two independent experiments is shown. (B) Immunoblot analysis of indicated proteins from HEK293T cells transiently transfected with indicated siRNA and FLAG-tagged RIT1 constructs or EV control. One of two independent experiments is shown. (C) In vitro MEK1 phosphorylation (Ser²¹⁸/Ser²²²) by RAF1 or BRAF protein isolated from HEK293T cells coexpressing RIT1^A57G, KRAS^Q61L, or an EV control. a.u., arbitrary units. Data points indicate the means ± SEM of four (RAF1) or three (BRAF) biological replicates (independently isolated RAF protein samples). p, phospho.