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. 2023 Jul 14;9(28):eadf4766. doi: 10.1126/sciadv.adf4766

Fig. 2. RIT1 associates with the PM through its HVR.

Fig. 2.

(A) SPR analysis with increasing concentrations of POPS-containing liposomes showing RIT1 (amino acids 17 to 219) association with negatively charged lipids. (B) SPR affinity curves showing relative binding affinity of RIT1 to lipid constructs. (C) RIT1 C-terminal amino acid sequence. PBRs are colored blue. (D) Live-cell confocal images of HeLa cells transiently transfected with indicated GFP-RIT1 constructs. Stable expression of mCherry-KRAS4B was used as a PM marker. Representative images from one of three independent experiments (n = 3). Scale bars, 15 μm. RU, response units.