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. Author manuscript; available in PMC: 2024 Mar 16.
Published in final edited form as: Cell. 2023 Mar 16;186(6):1127–1143.e18. doi: 10.1016/j.cell.2023.02.021

Figure 5: Tex-int are present at higher levels in the blood following ICB.

Figure 5:

A) Overview of cohort. Blood samples were collected pre-treatment (baseline, 2–29 days before surgery), at time of surgery, and at follow-up (27–38 days post-surgery) from patients with HNSCC that received anti-PD-L1 treatment prior to surgery (n = 10). Additionally, tumor samples were collected at time of surgery. For a few patients, additional samples were collected (see complete overview in Figure S6A). Samples were analyzed by mass cytometry. B) Heatmap of markers used for CD8+ T cell clustering. Scaled median expression per marker is shown for cluster annotation. C) UMAP of non-naïve CD8+ T cell clusters and expression for a subset of markers. D) Frequencies of non-naïve CD8+ T cell clusters. E) Paired differential abundance analysis of non-naïve CD8+ T cell subsets between blood at time of surgery and baseline (n = 10) (generalized linear mixed models). The log2 fold changes are plotted against the negative log10(nominal p-values). Colors indicate if cell populations are significantly more abundant in blood at time of surgery (purple) or baseline (blue) or not differentially abundant (False, grey) after Benjamini-Hochberg correction, FDR < 0.1. F) Cluster 5 and 9 abundances (as percentage of non-naïve CD8+ T cells) in paired blood samples at time of surgery and baseline. P-values obtained by generalized linear mixed models. G) Paired differential abundance analysis of non-naïve CD8+ T cell subsets between blood at time of follow-up and at baseline (n = 7) (generalized linear mixed models). See color scheme for Figure 6C. H) Cluster 2, 5, and 9 abundances (as percentage of non-naïve CD8+ T cells) in paired blood samples at follow-up and baseline. P-values obtained by generalized linear mixed models. I-J) Percentage of cluster 2 and 5 proliferating cells in (I) paired blood samples at time of surgery and baseline and (J) in blood and tumor at time of surgery. P-values obtained by paired Wilcoxon Rank Sum Test. K) Spearman correlation between cluster 16 and 18 in LN (MIBI data) and cluster 5 and 9 in blood (CyTOF data) at time of surgery. See also Figure S5.