(A) Immunofluorescence images of TurboGFP, TdTomato, and TNNT2 expression at day 30 of cardiac differentiation for WTC and SCVI-111 reporter lines. (B) RT-qPCR profiling of FHF markers HAND1 and TBX5 along with Cre Recombinase between day 0 and 30 of differentiation. (C) RT-qPCR profiling of pan-cardiac progenitor marker ISL1, and SHF markers TBX1 and FGF8 between day 030 of differentiation. (D) RT-qPCR profiling of pan-cardiomyocyte marker TNNT2. (E) RT-qPCR profiling of ventricular marker myosin light chain-2 (MYL2). N=3–4 biological replicates per timepoint. Significance determined by one-way ANOVA with Dunnet’s multiple comparisons correction. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Error bars represent standard error of the mean (SEM).
Figure 3—source data 1. qPCR human primer sequences.