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. 2023 Jul 17;133(14):e164660. doi: 10.1172/JCI164660

Figure 6. DNJ protects mitochondrial cristae morphology in HCM cybrids.

Figure 6

(A) Mitochondrial networks of control cybrids, HCM cybrids and HCM cybrids with DNJ. Cybrids were immunolabeled for the mitochondrial marker TOM20. Scale bar, 20 μm. (B) Representative TEM recordings of cybrids. Scale bar for left images, 2 μm. Scale bar for enlarged images, 200 nm. (C) Quantification of the overall cristae morphology on TEM recordings. (D) Quantification of mitochondrial cristae number on TEM recordings. Con: n = 47, HCM: n = 61, HCM + DNJ: n = 45 biologically independent mitochondrion. Values represent the mean ± SEM. 1-way ANOVA followed by Tukey’s test. **P < 0.01, ***P < 0.001. (E) Diameter of cristae. Con: n = 241, HCM: n = 160, HCM + DNJ: n = 195 biologically independent mitochondrion. Values represent the mean ± SEM. 1-way ANOVA followed by Tukey’s test. ***P < 0.001. (F) CJ frequency on TEM recordings. The number of CJs was manually determined and normalized to the cristae number. Con: n = 47, HCM: n = 61, HCM + DNJ: n = 45 biologically independent mitochondrion. Values represent the mean ± SEM. 1-way ANOVA followed by Tukey’s test. **P < 0.01, ***P < 0.001.