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. 2023 Jul 14;10(3):172–187. doi: 10.1055/s-0043-1771193

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The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ )

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Workflow concerning the analysis of cfDNA on enrolled patients. The NGS analysis, performed with Panels 1 and 2, is executed on the circulating DNA of the first sampling at time zero (T0). The sequential collecting starts from the seventh month to monitor the variants of clinical significance found in the NGS analysis. The quantification of cfDNA is performed on all liquid biopsies. cfDNA, circulating free DNA; ddPCR, droplet digital polymerase chain reaction; NGS, next-generation sequencing.