Table 7.
Detection of P. falciparum and P. vivax in real mixed infection templates.
| Artificial blood mixed-infection | Respective parasitaemia | Cycle threshold ± standard deviation | ||
|---|---|---|---|---|
| Duplex-PCR reagents | Simplex reagents | |||
| P. falciparum | P. vivax | |||
| P. falciparum–P. vivax | 100 p/µl–100 p/µl | 31.0 ± 0.3 | 31.5 ± 0.3 | |
| P. falciparum–P. vivax | 100 p/µl–10 p/µl | 29.5 ± 0.4 | 33.0 ± 0.9 | |
| P. falciparum–P. vivax | 100 p/µl–1 p/µl | 29.5 ± 0.5 | – | |
| P. falciparum–P. vivax | 10 p/µl–100 p/µl | 32.0 ± 0.3 | 30.1 ± 0.2 | |
| P. falciparum–P. vivax | 10 p/µl–10 p/µl | 32.3 ± 0.4 | 32.0 ± 0.1 | |
| P. falciparum- P. vivax | 10 p/µl–1 p/µl | 32.0 ± 0.1 | 37.0 ± 0.3 | |
| P. falciparum–P. vivax | 1 p/µl–100 p/µl | 41.5 ± 1.1 | 29.0 ± 0.3 | |
| P. falciparum- P. vivax | 1 p/µl–10 p/µl | 35.0 ± 0.3 | 32.0 ± 1.5 | |
| P. falciparum–P. vivax | 1 p/µl–1 p/µl | 34.5 ± 0.3 | 36.3 ± 0.6 | |
| P. falciparum | 100 p/µl | 30.2 ± 0.5 | – | 30.0 ± 0.4 |
| P. falciparum | 10 p/µl | 33.0 ± 0.6 | – | 33.0 ± 0.0 |
| P. falciparum | 1 p/µl | 36.2 ± 0.6 | – | 36.4 ± 1.5 |
| P. vivax | 100 p/µl | – | 30.2 ± 1.3 | 29.4 ± 0.1 |
| P. vivax | 10 p/µl | – | 31.1 ± 0.2 | 31.0 ± 0.1 |
| P. vivax | 1 p/µl | – | 36.0 ± 0.9 | 35.0 ± 0.5 |