Table 8.
Primers and probes used for identification of Plasmodium species targeting 18S genes.
| Species | Primer or probe | Sequence 5′–3′ | Fluorescent label | Reference |
|---|---|---|---|---|
| Duplex | ||||
| P. falciparum | Fal-F | CCGACTAGGTGTTGGATGAAAGTGTTAA | VIC-MGBNFQ | Shokoples et al.12,18 |
| Fal-R | AACCCAAAGACTTTGATTTCTCATAA | |||
| Fal probe | AGCAATCTAAAAGTCACCTCGAAAGATGACT | |||
| P. vivax | Viv-F | CCGACTAGGCTTTGGATGAAAGATTTTA | NED-MGBNFQ | |
| Viv-R | AACCCAAAGACTTTGATTTCTCATAA | |||
| Viv probe | AGCAATCTAAGAATAAACTCCGAAGAGAAAATT | |||
| Triplex | ||||
| P. malariae | Mal-F | CCGACTAGGTGTTGGATGATAGAGTAAA | FAM-MGBNFQ | Shokoples et al.12,18 |
| Mal-R | AACCCAAAGACTTTGATTTCTCATAA | |||
| Mal probe | CTATCTAAAAGAAACACTCAT | |||
| P. ovale | Ova-F | CCGACTAGGTTTTGGATGAAAGATTTTT | VIC-MGBNFQ | |
| Ova-R | AACCCAAAGACTTTGATTTCTCATAA | |||
| Ova probe | CGAAAGGAATTTTCTTATT | |||
| P. knowlesi | Knw-F | CTAAAATGCGCACAAAGTCGAT | NED-MGBNFQ | De Canale et al.13 |
| Knw-R | GCAGTTAAAACGCTCGTAGTTGAA | |||
| Knw probe-1 | CGGAGGCATCAGTTAT | |||
| Simplex | ||||
| Plasmodium spp. | Spp-F | AGCTCTTTCTTGATTTCTTGG | VIC-MGBNFQ | Hassanpour et al.14 |
| Spp-R | CAGACAAATCATATTCACGAACT | |||
| Spp probe-1 | AAACGGCCATGCATCACCAT | |||
| Simplex | ||||
| Human β2-megaglobulin | 2βMG-F | TGAGTATGCCTGCCGTGTGA | FAM-MGBNFQ | Shokoples et al.12,18 |
| 2βMG-R | ACTCATACACAACTTTCAGCAGCTTAC | |||
| 2βMG probe | CCATGTGACTTTGTCACAGCCCAAGATAGTT | |||