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. 2023 Jul 14;14:4218. doi: 10.1038/s41467-023-39955-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Quantification of the number of aBocs per cycle in adult animals of the indicated genotypes. Data are presented as mean values ± SEM. n = 6, 6, 5, 5 independent animals. ***P < 0.001 in one-way ANOVA with Bonferroni’s correction for multiple comparisons; n.s. not significant. b Quantification of the number of calcium spikes observed per cycle in hmc in adult animals of the indicated genotypes. “hmc frpr-21” denotes expressing frpr-21 cDNA under the nmur-3(Δ) promoter. “Pflp-9 flp-9” denotes expressing flp-9 cDNA under a 4.8 kb flp-9 promoter fragment. “GABAergic flp-9” denotes expressing flp-9 cDNA under the unc-47 promoter. Wild-type: 33 cycles in 8 animals, frpr-21: 23 cycles in 9 animals, frpr-17: 56 cycles in 13 animals, frpr-21; frpr-17: 32 cycles in 10 animals, frpr-21; frpr-17; hmc frpr-21: 38 cycles in 7 animals, flp-9; frpr-17: 47 cycles in 8 animals, frpr-21; flp-9; frpr-17: 44 cycles in 7 animals. ***P < 0.001 and *P < 0.05 in two-sided chi-square test with Bonferroni’s correction for multiple comparisons; n.s. not significant. c Left: average traces of calcium dynamics in hmc aligned to the calcium spike initiation time in the indicated mutants. The solid lines indicate average fold change in GCaMP intensity and the shades indicate SEM. Right: quantification of the average peak amplitude, rise time, and half-decay time from the traces on the left. Data are presented as mean values ± SEM. n = 10, 9, 5, 6, 6, 5 cycles from different animals. **P < 0.01 and *P < 0.05 in one-way ANOVA with Bonferroni’s correction for multiple comparisons; n.s. not significant. d Representative image of hmc cell body (arrow) and processes from adults expressing FRPR-21::GFP in hmc under the nmur-3(Δ) promoter. Similar expression patterns were observed in all 15 animals examined. Scale bar, 40 μm. e Quantification of the number of aBocs per cycle in adult animals of the indicated genotypes. “flp-9 (OE)” denotes expression of flp-9 cDNA under the GABAergic-specific unc-47 promoter. Data are presented as mean values ± SEM. n = 6, 3, 3, 4 independent animals. **P < 0.01 in one-way ANOVA with Bonferroni’s correction for multiple comparisons; n.s. not significant. f Quantification of the number of calcium spikes observed per cycle in hmc in adult animals of the indicated genotypes. “frpr-21 (OE)” denotes expressing frpr-21 cDNA in hmc using the nmur-3(Δ) promoter. Wild-type: 33 cycles in 8 animals, flp-9: 27 cycles in 8 animals, flp-9 (OE): 27 cycles in 5 animals, frpr-21; flp-9 (OE): 23 cycles in 5 animals, frpr-21 (OE): 46 cycles in 7 animals, frpr-21; frpr-21 (OE): 29 cycles in 8 animals. ***P < 0.001 in two-sided chi-square test with Bonferroni’s correction for multiple comparisons; n.s. not significant.