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. 2023 Jul 14;14(7):431. doi: 10.1038/s41419-023-05951-5

Fig. 2. Screening for highly metastatic subclones in vivo to identify the candidate genes.

Fig. 2

A Enhanced metastatic capacity of the mutagenic libraries in vivo, representative images were shown. Upper: organs from mice transplanted with control cells Pan02-4B3. Lower: organs from mice transplanted with the library. B Anxa3 was identified by Splinkerette PCR from the metastatic lesions. Target bands were extracted and sequenced. C Sequence alignment in UCSC Blat for Anxa3. PB-GSV initiated an antisense RNA which was opposite to the direction of Anxa3 transcription. D Overall survival time was prolonged when intraperitoneally transplanting GSV-Anxa3 subclone into mice with Dox treatment (+Dox) as compared to the control group (−Dox) (n = 10, *p < 0.05, Kaplan–Meier survival analysis). E Macro-metastatic lung nodules decreased when subcutaneously transplanting GSV-Anxa3 subclone into mice with Dox treatment (+Dox) as compared to the control group (−Dox). Mice were sacrificed on day 55 (n = 10, *p < 0.05).