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. 2023 Jul 14;14(7):431. doi: 10.1038/s41419-023-05951-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Heat map of the DEGs identified by RNA-seq in Pan02 control cells (Yw-WT) and YWHAZ overexpression cells (Yw-OV) (>2-fold, p < 0.05, n = 3). B Top enriched GO terms in downregulated genes. C Western blot analysis of the EMT biomarkers and transcription factors, Ywhaz was cloned with a fusion HA-tag and anti-HA shows the expression of YWHAZ. D Hematoxylin-eosin staining and immunohistochemical staining of the EMT biomarkers and transcription factors in subcutaneous tumors. E-cadherin and ZO-1 not only showed a difference in intensity but also a change in location after YWHAZ overexpression (n = 3, p < 0.05).