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. 2023 Jul 14;13:11418. doi: 10.1038/s41598-023-38638-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adequate fracture union in HCS mice. (a) Representative callus sections (Movat’s Pentachrome staining) of four exemplary types of osseous bridging to score fracture union 21 days after osteotomy: complete bridging (all four cortices bridged by callus), partial bridging (two to three cortices bridged by callus), incomplete bridging (callus present, but no bridging visible), and non-union (rounded cortices, minimal presence of callus). Images were captured with CellSens Standard V4.1, Olympus, https://www.olympus-lifescience.com/de/software/cellsens/Tokio, Japan. (b) Semi-quantitative scoring of osseous bridging in WT and HCS mice 21 days after osteotomy. n = 6 mice per group.