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. 2023 Jul 14;13:11418. doi: 10.1038/s41598-023-38638-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impaired biomechanical stability of healed femora in HCS mice. (a) Representative µCT images of the healing femur of WT and HCS mice 28 days after osteotomy. Upper row: overview. Lower row: detailed image of callus. Quantification of bone volume (BV) and tissue volume (TV) in the same mice. Images were generated by SCANCO Medical microCT Analysis Software V6/10–2009, https://www.scanco.ch/images/Brochures/microct_sw_v8.pdf, Brüttisellen, Switzerland (b) Quantification of mechanical properties of the healing femora as determined by three-point-bending test in the same animals. F-Max = maximum force that the bone could withstand; Young’s modulus = stiffness along the axis. Non-parametric Mann–Whitney U test, **p < 0.005. Data are shown as mean with standard deviation. Data points indicate individual measurements from each mouse.