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. 2023 Jul 14;13:11397. doi: 10.1038/s41598-023-38280-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Chromatin restoration in CA-nucleosome deposition mutants is independent of cell cycle arrest. (A) Plasmid topoisomer distribution of the 2µ plasmid in wild type, cdc20-3, cac1∆ rtt106∆ and cac1∆ rtt106 cdc20-3 cells at 34 °C. Cells were synchronized in G1, released into fresh medium for 60 min and resynchronized in G1. (B) Plasmid topoisomer distribution of the 2µ plasmid in wild type, t::HHF2, mad2∆ and t::HHF2 mad2∆ cells synchronized in G1 and released into fresh medium for different times. Samples were run into different gels due to space limitations, and processed in parallel. Cell cycle progression and topoisomer profiles are shown. r and SC(−) indicate relaxed and negative supercoiling, respectively. Cropped images show only relaxed and negatively supercoiled topoisomers. Original gels are presented in Fig. S3. The experiments were repeated at least twice with similar results.