Fig. 6. ZYA is a robust gate opening compound that can prevent inhibition by soluble oligomers.
a. 20S proteasome (0.5 nM) activity (nLPnLD-amc hydrolysis, rfu/min) with ZYA at the indicated concentrations, with and without Aβ oligomers (0.5 μM) oligomers21. Rate of nLPnLD-amc hydrolysis is normalized to the control. b. 20S proteasomes incubated with and without α-Syn (top), and with or without ZYA (1 mM). Rate of nLPnLD-amc hydrolysis is normalized to the control. c. Same as b except with HttQ53 oligomers, Rate of nLPnLD-amc hydrolysis is normalized to the control. Data (means) is representative of three or more independent experiments each performed in triplicate. Error bars represent ± standard deviation.