Table 1.

Regression and validation data for the determination of SER.DMP and DMP by the proposed methods

Drug Name	SER.DMP
Parameters	HPLC method	TLC densitometric method
Wavelength (nm)	220 nm	220 nm
Linearity range	2.5— 25 (μg/mL)	5— 25 (μg/spot)
LOD	0.051(μg/mL)	0.184 (μg/spot)
LOQ	0.165 (μg/mL)	0.202 (μg/spot)
Regression Equation	y a = 0.0989 C b + 0.0331	y a = 0.0824 C b + 0.0216
Correlation coefficient (r)	0.9998	0.9997
Accuracy (% R) ± SD	100.43 ± 1.02	99.68 ± 1.03
Precision (% RSD)
Repeatability c	1.021	1.025
Intermediate precision d	1.015	1.039
Robustness (% RSD)
Mobile phase contents ratio (± 2%)	0.812	1.386
Detection wavelengths (± 2 nm)	1.349	1.526
Flow rate (± 0.1 ml/min.)	1.778	ـــــــ

Drug Name	DMP
Parameters	HPLC method	TLC densitometric method
Wavelength (nm)	220 nm	220 nm
Linearity range	2.5–25 (μg/mL)	5–25 (μg/spot)
LOD	0.098 (μg/mL)	0.115 (μg/spot)
LOQ	0.186 (μg/mL)	0.237 (μg/spot)
Regression Equation	y a = 0.0897 C b + 0.0522	y a = 0.0905 C b + 0.0612
Correlation coefficient (r)	0.9997	0.9996
Accuracy (% R) ± SD	100.15 ± 1.1	99.50 ± 1.2
Precision (% RSD)
Repeatability c	1.105	1.160
Intermediate precision d	1.251	1.249
Robustness (% RSD)
Mobile phase contents ratio (± 2%)	0.812	1.128
Detection wavelengths (± 2 nm)	1.349	1.630
Flow rate (± 0.1 ml/min.)	1.778	ـــــــ

^a Peak area of mixture of SER.DMP and DMP

^b Concentration in μg/ml for HPLC method and in μg/spot for TLC densitometric method

^c The intraday (n = 3), average of three concentrations of SER.DMP and DMP (10, 15 and

20 μg/mL) for HPLC method and (10,15 and 20 μg/spot) for TLC densitometric method

repeated three times within the day

^d The interday (n = 3), average of three concentrations of SER.DMP and DMP (10,15

and 20 μg/mL) for HPLC method and (10,15 and 20 μg/spot) for TLC densitometric

method repeated three times in three days