Fig. 3.

FLU inhibits PD-1 expression in Jurkat T cells. A Western blotting was performed with extracts of Jurkat T cells treated with FLU (10 µM) or vehicle for 24 h. Membranes were treated with antibodies to PD-1 or β-actin then with secondary antibodies for detection. B Alternatively, cells treated with FLU (10 µM) or vehicle for 24 h were tested for PD-1 immunopositivity by flow cytometry. Data is representative of 3 biologically-independent experiments. Error bars represent SEM. Statistical significance was assessed by t test