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. 2023 Jul 12;11:e15449. doi: 10.7717/peerj.15449

Table 1. Ingredient and proximate composition of experimental diets (% dry matter basis).

Calcium source/Calcium percentage
Ingredients 0% ES 3% ES 6% ES 6% SS 9% ES
aSoybean meal 61.5 61.5 61.5 61.5 61.5
bCorn starch 25 22 19 19 16
cCMC 1.5 1.5 1.5 1.5 1.5
dVitamin premix 3 3 3 3 3
eMineral premix 2 2 2 2 2
fPalm oil 7 7 7 7 7
gEggshell 0 3 6 0 9
hSeashell 0 0 0 6 0
Proximate composition
Moisture 12.2 12.4 12.3 12.4 12.2
Crude protein 30.3 30.1 30.3 30.4 30.2
Lipid 8.1 8.1 7.9 8.2 7.8
Ash 10.8 11.2 11.3 10.7 10.5

Notes:

a

GENTIDE fermented soybean, China.

b

STAR brand. Giant Sdn. Bhd.

c

Carboxymethyl cellulose (CMC), Sigma.

d

Vitamin mixture (g/kg mixture): Ascorbic acid, 45.0; Inositol, 8.0; Choline chloride, 75.0; Niacin, 4.5; Riboflavin, 1.0; Pyridoxine HCL, 1.0; Thiamine HCL, 0.92Retinyl acetate, 0.60; Vitamin D3, 0.083; Menadione, 1.67; DL alpha tocopherol acetate, 8.0; D-biotin, 0.02; Folic acid, 0.09; Vitamin B12, 0.00135.

e

Mineral mixture (g/kg mixture): 327.0; Ferrous sulphate, 25.0; Magnesium sulphate, 132.0; Potassium chloride, 50.0; Sodium chloride, 60.0; Potassium iodide, 0.15; Copper sulphate, 0.785; Manganese oxide, 0.8; Cobalt carbonate, 1.0; Zinc oxide, 3.0; Sodium salenite, 0.011.

f

Sri Murni Brand Palm Oil.

g

Eggshells collected from local restaurants, Restaurant Jamal Saleem.

h

Seashells collected from the local beach, Tanjung Aru Beach.